BMC Microbiology | |
Molecular epidemiology of extended-spectrum beta-lactamase-producing Escherichia coli in Tunisia and characterization of their virulence factors and plasmid addiction systems | |
Research Article | |
Nathalie Genel1  Guillaume Arlet1  Basma Mnif2  Hela Harhour3  Adnene Hammami3  Faouzia Mahjoubi3  Jihène Jdidi4  | |
[1] Faculté de Médecine, Site Saint-Antoine, Laboratoire de Bactériologie, Université Pierre et Marie Curie-Paris-6, Paris, France;Laboratoire de Microbiologie, Hôpital Habib Bourguiba, Sfax, Tunisie;Faculté de Médecine, Site Saint-Antoine, Laboratoire de Bactériologie, Université Pierre et Marie Curie-Paris-6, Paris, France;Laboratoire de Microbiologie-Faculté de Médecine de Sfax, Avenue Majida Boulila, 3027, Sfax, Tunisie;Laboratoire de Microbiologie, Hôpital Habib Bourguiba, Sfax, Tunisie;Laboratoire de Microbiologie-Faculté de Médecine de Sfax, Avenue Majida Boulila, 3027, Sfax, Tunisie;Service de médecine préventive, Hôpital Hédi Chaker, Sfax, Tunisie; | |
关键词: E. coli; ESBL; CTX-M-15; Plasmid; Addiction systems; Virulence; | |
DOI : 10.1186/1471-2180-13-147 | |
received in 2013-03-28, accepted in 2013-05-27, 发布年份 2013 | |
来源: Springer | |
【 摘 要 】
BackgroundExtended-spectrum β-lactamases (ESBLs), particularly CTX-M- type ESBLs, are among the most important resistance determinants spreading worldwide in Enterobacteriaceae. The aim of this study was to characterize a collection of 163 ESBL-producing Escherichia coli collected in Tunisia, their ESBL-encoding plasmids and plasmid associated addiction systems.ResultsThe collection comprised 163 ESBL producers collected from two university hospitals of Sfax between 1989 and 2009. 118 isolates harbored blaCTX-M gene (101 blaCTX-M-15 gene and 17 blaCTX-M-14 gene). 49 isolates carried blaSHV-12 gene, 9 blaSHV-2a gene and only 3 blaTEM-26 gene. 16 isolates produced both CTX-M and SHV-12. The 101 CTX-M-15-producing isolates were significantly associated to phylogroup B2 and exhibiting a high number of virulence factors. 24 (23.7%) of the group B2 isolates belonged to clonal complex ST131. Pulsed-field gel electrophoresis (PFGE) typing revealed a genetic diversity of the isolates. 144 ESBL determinants were transferable mostly by conjugation. The majority of plasmid carrying blaCTX-M-15 genes (72/88) were assigned to various single replicon or multireplicon IncF types and had significantly a higher frequency of addiction systems, notably the VagCD module.ConclusionThis study demonstrates that the dissemination of CTX-M-15 producing E. coli in our setting was due to the spread of various IncF-type plasmids harboring multiple addiction systems, into related clones with high frequency of virulence determinants.
【 授权许可】
CC BY
© Mnif et al.; licensee BioMed Central Ltd. 2013
【 预 览 】
Files | Size | Format | View |
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RO202311097908636ZK.pdf | 538KB | download |
【 参考文献 】
- [1]
- [2]
- [3]
- [4]
- [5]
- [6]
- [7]
- [8]
- [9]
- [10]
- [11]
- [12]
- [13]
- [14]
- [15]
- [16]
- [17]
- [18]
- [19]
- [20]
- [21]
- [22]
- [23]
- [24]
- [25]
- [26]
- [27]
- [28]
- [29]
- [30]
- [31]
- [32]
- [33]