The elicitation of a systemic resistance by Pseudomonas putidaBTP1 in tomato involves the stimulation of two lipoxygenase isoforms

BMC Plant Biology

Research Article

Charlotte Bureau¹  Jacques Dommes¹  Martin Mariutto¹  Francéline Duby¹  Marie-Laure Fauconnier²  Marc Ongena³  Akram Adam⁴  Philippe Thonart⁵ 

[1] Laboratory of Plant Molecular Biology and Biotechnology, Faculty of Sciences, Department of Life Sciences, University of Liège, Boulevard du Rectorat, 27, Liège, Belgium;Plant Biology Unit, Gembloux Agro-Bio Tech, University of Liège, Avenue de la Faculté, 2A, Gembloux, Belgium;Walloon Centre of Industrial Biology, Gembloux Agro-Bio Tech, University of Liège, Passage des Déportés, 2, Gembloux, Belgium;Walloon Centre of Industrial Biology, University of Liège, Boulevard du Rectorat, 29, Liège, Belgium;Walloon Centre of Industrial Biology, University of Liège, Boulevard du Rectorat, 29, Liège, Belgium;Walloon Centre of Industrial Biology, Gembloux Agro-Bio Tech, University of Liège, Passage des Déportés, 2, Gembloux, Belgium;

关键词: Linolenic Acid;    Plant Growth Promote Rhizobacteria;    Induce Systemic Resistance;    Pathogen Inoculation;    Systemic Acquire Resistance;   

DOI  :  10.1186/1471-2229-11-29

 received in 2010-03-09, accepted in 2011-02-04,  发布年份 2011

来源: Springer

【 摘 要 】

BackgroundSome non-pathogenic rhizobacteria called Plant Growth Promoting Rhizobacteria (PGPR) possess the capacity to induce in plant defense mechanisms effective against pathogens. Precedent studies showed the ability of Pseudomonas putida BTP1 to induce PGPR-mediated resistance, termed ISR (Induced Systemic Resistance), in different plant species. Despite extensive works, molecular defense mechanisms involved in ISR are less well understood that in the case of pathogen induced systemic acquired resistance.ResultsWe analyzed the activities of phenylalanine ammonia-lyase (PAL) and lipoxygenase (LOX), key enzymes of the phenylpropanoid and oxylipin pathways respectively, in tomato treated or not with P. putida BTP1. The bacterial treatment did not stimulate PAL activity and linoleate-consuming LOX activities. Linolenate-consuming LOX activity, on the contrary, was significantly stimulated in P. putida BTP1-inoculated plants before and two days after infection by B. cinerea. This stimulation is due to the increase of transcription level of two isoforms of LOX: TomLoxD and TomLoxF, a newly identified LOX gene. We showed that recombinant TomLOXF preferentially consumes linolenic acid and produces 13-derivative of fatty acids. After challenging with B. cinerea, the increase of transcription of these two LOX genes and higher linolenic acid-consuming LOX activity were associated with a more rapid accumulation of free 13-hydroperoxy-octadecatrienoic and 13-hydroxy-octadecatrienoic acids, two antifungal oxylipins, in bacterized plants.ConclusionIn addition to the discovery of a new LOX gene in tomato, this work is the first to show differential induction of LOX isozymes and a more rapid accumulation of 13-hydroperoxy-octadecatrienoic and 13-hydroxy-octadecatrienoic acids in rhizobacteria mediated-induced systemic resistance.

【 授权许可】

Unknown   
© Mariutto et al; licensee BioMed Central Ltd. 2011. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.