Developmental iodine deficiency and hypothyroidism impair neural development in rat hippocampus: involvement of doublecortin and NCAM-180

BMC Neuroscience

Research Article

Jie Chen¹  Wei Wei¹  Hongde Xu¹  Jiapeng Zhong¹  Jing Dong¹  Jian Gong¹  Yi Wang¹  Wanyang Liu¹  Qi Xi² 

[1] Department of Occupational and Environmental Health, School of Public Health, China Medical University, Shenyang, PR China;Department of Occupational and Environmental Health, School of Public Health, China Medical University, Shenyang, PR China;Department of Physiology, the University of Tennessee Health Science Center, 38163, Memphis, TN, USA;

关键词: Thyroid Hormone;    Nerve Fiber;    Hypothyroidism;    Dentate Gyrus;    Iodine Deficiency;   

DOI  :  10.1186/1471-2202-11-50

 received in 2009-12-17, accepted in 2010-04-23,  发布年份 2010

来源: Springer

【 摘 要 】

BackgroundDevelopmental iodine deficiency results in inadequate thyroid hormone (TH), which damages the hippocampus. Here, we explored the roles of hippocampal doublecortin and neural cell adhesion molecule (NCAM)-180 in developmental iodine deficiency and hypothyroidism.MethodsTwo developmental rat models were established with either an iodine-deficient diet, or propylthiouracil (PTU)-adulterated water (5 ppm or 15 ppm) to impair thyroid function, in pregnant rats from gestational day 6 until postnatal day (PN) 28. Silver-stained neurons and protein levels of doublecortin and NCAM-180 in several hippocampal subregions were assessed on PN14, PN21, PN28, and PN42.ResultsThe results show that nerve fibers in iodine-deficient and 15 ppm PTU-treated rats were injured on PN28 and PN42. Downregulation of doublecortin and upregulation of NCAM-180 were observed in iodine-deficient and 15 ppm PTU-treated rats from PN14 on. These alterations were irreversible by the restoration of serum TH concentrations on PN42.ConclusionDevelopmental iodine deficiency and hypothyroidism impair the expression of doublecortin and NCAM-180, leading to nerve fiber malfunction and thus impairments in hippocampal development.

【 授权许可】

Unknown   
© Gong et al; licensee BioMed Central Ltd. 2010. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.