Phytochemical compositions of extract from peel of hawthorn fruit, and its antioxidant capacity, cell growth inhibition, and acetylcholinesterase inhibitory activity

BMC Complementary and Alternative Medicine

Research Article

Zhaojie Ji¹  Weidong Chen¹  Panpan Wu²  Bin Yang³  Jianyong Zhang⁴  Fajie Li⁵ 

[1] Anhui University of Chinese Medicine, 230012, Hefei, China;Anhui University of Chinese Medicine, 230012, Hefei, China;Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, No. 16 Nanxiaojie, Dongzhimennei Ave., Dongcheng District, 100700, Beijing, China;Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, No. 16 Nanxiaojie, Dongzhimennei Ave., Dongcheng District, 100700, Beijing, China;Pharmacy School, Zunyi Medical University, 563000, Guizhou, China;State Key Laboratory of Dao-di Herbs, National Resource Center for Chinese Materia Medica, China Academy of Chinese Medical Sciences, 100700, Beijing, China;Flow Station of Post-Doctoral Scientific Research, China Academy of Chinese Medical Sciences, 100700, Beijing, China;

关键词: Hawthorn peel;    Macroporous resin extract;    Phytochemical composition;    Antioxidant capacity;    Cell growth inhibition;    AChE inhibitory activity;   

DOI  :  10.1186/s12906-017-1662-y

 received in 2016-12-07, accepted in 2017-03-07,  发布年份 2017

来源: Springer

【 摘 要 】

BackgroundHawthorn fruit (HF) is a well-known traditional medicine in China with the effects of improving digestion and regulating qi-flowing for removing blood stasis. Modern pharmacological experiments showed that HF extract has various pharmaceutical properties and flavonoids are considered as the main bioactive compounds. In this paper, Diaion HP-20 adsorption chromatography was used to enrich flavonoids in PHF, and the phytochemical composition of EPHF was analyzed by high performance liquid chromatography (HPLC) and liquid chromatography tandem mass spectrometry (LC-MS). In addition, EPHF’s antioxidant capacity, acetylcholinesterase (AChE) inhibitory activity and cytotoxic activity were evaluated.MethodsEPHF was obtained by Diaion HP-20 adsorption chromatography. Phytochemical composition of EPHF was analyzed qualitatively and quantitatively using HPLC and LC-MS. Radical scavenging capacity of EPHF was estimated using 2,2-diphenyl-1-picryhydrazyl (DPPH) assay and oxygen radical absorbance capacity (ORAC) assay. The AChE inhibitory activity of EPHF was evaluated by Ellman method. Cytotoxic activity of EPHF was assessed by means of MTT assay.ResultsEight kinds of components were identified, in which ideain with the value of 179.4 mg/g was identified to be present in the highest level in EPHF, followed by (−)-epicatechin, chlorogenic acid, cyanidin 3-arabinoside, hyperoside and isoquercitrin at the concentrations of 40.9, 10.0, 1.4, 0.4 and 0.2 mg/g, respectively. The contents of these compounds in EPHF were much higher than those in PHF and HF. In addition, EPHF exhibited strong antioxidant and AChE inhibitory activity (ORAC value: 11.65 ± 2.37 μM Trolox equivalents (TE)/mg, DPPH IC50 value: 6.72 μg/mL, anti-AChE activity IC50 value: 11.72 μg/mL) compared with PHF and HF. Moreover, EPHF exhibited high levels of cytotoxicity on MCF-7 and SKOV-3 human tumour cell lines in a dose-dependent manner with the IC50 of 2.76 and 80.11 μg/mL, respectively.ConclusionsMacroporous resin is useful for the extraction and separation of the total flavonoids from PHF. The contents of flavonoids especially anthocyanin in EPHF were increased significantly compared with the PHF, and EPHF exhibited strong antioxidant, AChE inhibitory activity and cytotoxicity on human tumour cells.

【 授权许可】

CC BY   
© The Author(s). 2017