Metabolite profiling and network analysis reveal coordinated changes in grapevine water stress response

BMC Plant Biology

Research Article

Asfaw Degu¹  Uri Hochberg¹  Zoran Nikoloski²  Shimon Rachmilevitch³  Tanya Gendler³  David Toubiana³  Aaron Fait³ 

[1] Albert Katz International School, Ben-Gurion University of the Negev, 84990, Sede Boqer, Israel;the French Associates Institute for Agriculture and Biotechnology of Drylands (FAAB), the Jacob Blaustein Institutes for Desert Research, Ben-Gurion University of the Negev, 84990, Sede Boqer, Israel;Max-Planck-Institut für Molekulare Pflanzenphysiologie, Am Mühlenberg 1, 14476, Potsdam, Golm, Germany;the French Associates Institute for Agriculture and Biotechnology of Drylands (FAAB), the Jacob Blaustein Institutes for Desert Research, Ben-Gurion University of the Negev, 84990, Sede Boqer, Israel;

关键词: Vitis vinifera;    Grapevine;    Metabolite profiling;    Water deficit response;    Stress physiology;   

DOI  :  10.1186/1471-2229-13-184

 received in 2013-06-30, accepted in 2013-11-06,  发布年份 2013

来源: Springer

【 摘 要 】

BackgroundGrapevine metabolism in response to water deficit was studied in two cultivars, Shiraz and Cabernet Sauvignon, which were shown to have different hydraulic behaviors (Hochberg et al. Physiol. Plant. 147:443–453, 2012).ResultsProgressive water deficit was found to effect changes in leaf water potentials accompanied by metabolic changes. In both cultivars, but more intensively in Shiraz than Cabernet Sauvignon, water deficit caused a shift to higher osmolality and lower C/N ratios, the latter of which was also reflected in marked increases in amino acids, e.g., Pro, Val, Leu, Thr and Trp, reductions of most organic acids, and changes in the phenylpropanoid pathway. PCA analysis showed that changes in primary metabolism were mostly associated with water stress, while diversification of specialized metabolism was mostly linked to the cultivars. In the phloem sap, drought was characterized by higher ABA concentration and major changes in benzoate levels coinciding with lower stomatal conductance and suberinization of vascular bundles. Enhanced suberin biosynthesis in Shiraz was reflected by the higher abundance of sap hydroxybenzoate derivatives. Correlation-based network analysis revealed that compared to Cabernet Sauvignon, Shiraz had considerably larger and highly coordinated stress-related changes, reflected in its increased metabolic network connectivity under stress. Network analysis also highlighted the structural role of major stress related metabolites, e.g., Pro, quercetin and ascorbate, which drastically altered their connectedness in the Shiraz network under water deficit.ConclusionsTaken together, the results showed that Vitis vinifera cultivars possess a common metabolic response to water deficit. Central metabolism, and specifically N metabolism, plays a significant role in stress response in vine. At the cultivar level, Cabernet Sauvignon was characterized by milder metabolic perturbations, likely due to a tighter regulation of stomata upon stress induction. Network analysis was successfully implemented to characterize plant stress molecular response and to identify metabolites with a significant structural and biological role in vine stress response.

【 授权许可】

Unknown   
© Hochberg et al.; licensee BioMed Central Ltd. 2013. This article is published under license to BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.