The gene-reduction effect of chromosomal losses detected in gastric cancers

BMC Gastroenterology

Research Article

Eun-Joo Seo¹  Eun-Jung Jeon²  Sang-Wook Choi²  Jung-Hwan Oh²  Seung-Jin Hong³  Mun-Gan Rhyu³ 

[1] Department of Clinical Pathology, College of Medicine, The Catholic University of Korea, Seoul, Korea;Department of Internal Medicine, College of Medicine, The Catholic University of Korea, Seoul, Korea;Department of Microbiology, College of Medicine, The Catholic University of Korea, Seoul, Korea;

关键词: Gastric Cancer;    Microsatellite Genotype;    Chromosomal Loss;    Cancer Progenitor Cell;    Small Gastric Cancer;   

DOI  :  10.1186/1471-230X-10-138

 received in 2010-07-02, accepted in 2010-11-20,  发布年份 2010

来源: Springer

【 摘 要 】

BackgroundThe level of loss of heterozygosity (LOH) that reduces a gene dose and exerts a cell-adverse effect is known to be a parameter for the genetic staging of gastric cancers. This study investigated if the cell-adverse effect induced with the gene reduction was a rate-limiting factor for the LOH events in two distinct histologic types of gastric cancers, the diffuse- and intestinal-types.MethodsThe pathologic specimens obtained from 145 gastric cancer patients were examined for the level of LOH using 40 microsatellite markers on eight cancer-associated chromosomes (3p, 4p, 5q, 8p, 9p, 13q, 17p and 18q).ResultsMost of the cancer-associated chromosomes were found to belong to the gene-poor chromosomes and to contain a few stomach-specific genes that were highly expressed. A baseline-level LOH involving one or no chromosome was frequent in diffuse-type gastric cancers. The chromosome 17 containing a relatively high density of genes was commonly lost in intestinal-type cancers but not in diffuse-type cancers. A high-level LOH involving four or more chromosomes tended to be frequent in the gastric cancers with intestinal and mixed differentiation. Disease relapse was common for gastric cancers with high-level LOH through both the hematogenous (38%) and non-hematogenous (36%) routes, and for the baseline-level LOH cases through the non-hematogenous route (67%).ConclusionsThe cell-adverse effect of gene reduction is more tolerated in intestinal-type gastric cancers than in diffuse-type cancers, and the loss of high-dose genes is associated with hematogenous metastasis.

【 授权许可】

Unknown   
© Hong et al; licensee BioMed Central Ltd. 2010. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.