The role of the two-component systems Cpx and Arc in protein alterations upon gentamicin treatment in Escherichia coli

BMC Microbiology

Research Article

Elke Hammer¹  Sabine Hunke²  Gianna Panasia³  Kristin Surmann⁴  Emina Ćudić⁴ 

[1] Department of Functional Genomics, Interfaculty Institute of Genetics and Functional Genomics, University Medicine Greifswald, Friedrich-Ludwig-Jahn-Straße 15A, 17475, Greifswald, Germany;FB 5 Microbiology, Department of Biology/Chemistry, University Osnabrück, Barbarastraße 11, 49076, Osnabrück, Germany;FB 5 Microbiology, Department of Biology/Chemistry, University Osnabrück, Barbarastraße 11, 49076, Osnabrück, Germany;Department of Biology, Institute of Molecular Microbiology and Biotechnology, Universität Münster, Corrensstraße 3, 48149, Münster, Germany;null;

关键词: E. coli;    Two - component system;    Cpx;    Arc;    Aminoglycosides;    Protein interactions;    Absolute quantification;    SRM;    Proteome profiling;   

DOI  :  10.1186/s12866-017-1100-9

 received in 2017-04-19, accepted in 2017-09-05,  发布年份 2017

来源: Springer

【 摘 要 】

BackgroundThe aminoglycoside antibiotic gentamicin was supposed to induce a crosstalk between the Cpx- and the Arc-two-component systems (TCS). Here, we investigated the physical interaction of the respective TCS components and compared the results with their respective gene expression and protein abundance. The findings were interpreted in relation to the global proteome profile upon gentamicin treatment.ResultsWe observed specific interaction between CpxA and ArcA upon treatment with the aminoglycoside gentamicin using Membrane-Strep-tagged protein interaction experiments (mSPINE). This interaction was neither accompanied by detectable phosphorylation of ArcA nor by activation of the Arc system via CpxA. Furthermore, no changes in absolute amounts of the Cpx- and Arc-TCS could be determined with the sensitive single reaction monitoring (SRM) in presence of gentamicin. Nevertheless, upon applying shotgun mass spectrometry analysis after treatment with gentamicin, we observed a reduction of ArcA ~ P-dependent protein synthesis and a significant Cpx-dependent alteration in the global proteome profile of E. coli.ConclusionsThis study points to the importance of the Cpx-TCS within the complex regulatory network in the E. coli response to aminoglycoside-caused stress.

【 授权许可】

CC BY   
© The Author(s). 2017