Uygur Medicine Xipayi Kui Jie’an Affects Gene Expression profiles in intestinal tissue Lesions in a Rat Model of Ulcerative Colitis

BMC Complementary and Alternative Medicine

Research Article

Jingping Zhang¹  Li Zhong¹  Mairipaiti Abudula¹  Kurexi Yunusi¹  Gulinisha Mosha¹  Ajiguli Nuermaimaiti¹  Halmurat Upur² 

[1] Department of Molecular Biology and Biochemistry, College of Basic Medicine, Xinjiang Medical University, 830000, Urumqi, Xinjiang, China;Traditional Uighur Medicine Institute, Xinjiang Medical University, 830000, Urumqi, Xinjiang, China;

关键词: Uygur Medicine;    Ulcerative colitis;    Xipayi kuijie’an;    Immunity and inflammation;    Microarray;   

DOI  :  10.1186/s12906-015-0672-x

 received in 2014-10-28, accepted in 2015-05-13,  发布年份 2015

来源: Springer

【 摘 要 】

BackgroundThe aim of this study was to investigate the mechanisms underlying the therapeutic effect of Uygur medicine KJA on UC in a rat model.MethodsUC was induced in Wistar rats by application of 2, 4-dinitrochlorobenzene and acetic acid and were then treated with three different doses of KJA, and normal saline as control. After treatment for 20 days, the gene expression profile of colonic tissue was analyzed by microarray and verified by quantitative real-time RT-PCR.ResultsAnimals treated with the three different doses of KJA were compared with normal saline controls, wherein microarray analysis identified 1991, 2163, and 1677 differentially expressed genes respectively, of which 444 genes were raised and 670 genes were decrease spliced together in the three doses tested. The KEGG pathway analyses found commonly raised genes related to several different biological functions. Interesting genes included TRL2, IL-1β, TGF-β1, and NF-κB were confirmed by quantitative PCR.ConclusionsThe therapeutic effect of KJA on UC is likely explained by specific effects on the expression of genes, which are the effector molecules known to be involved in the development of UC. Further studies on differentially expressed genes will help explain the mechanism of action of Uygur medicine KJA.

【 授权许可】

Unknown   
© Yunusi et al.; licensee BioMed Central. 2015. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.