Multi-host environments select for host-generalist conjugative plasmids

BMC Evolutionary Biology

Research Article

James P. J. Hall¹  Anastasia Kottara¹  Michael A. Brockhurst¹  Ellie Harrison¹ 

[1] Department of Biology, University of York, Wentworth Way, YO10 5DD, York, UK;

关键词: Broad Host Range;    Bacterial Host;    Antagonistic Pleiotropy;    Mercury Resistance;    Broad Host Range Plasmid;   

DOI  :  10.1186/s12862-016-0642-z

 received in 2015-12-08, accepted in 2016-03-22,  发布年份 2016

来源: Springer

【 摘 要 】

BackgroundConjugative plasmids play an important role in bacterial evolution by transferring ecologically important genes within and between species. A key limit on interspecific horizontal gene transfer is plasmid host range. Here, we experimentally test the effect of single and multi-host environments on the host-range evolution of a large conjugative mercury resistance plasmid, pQBR57. Specifically, pQBR57 was conjugated between strains of a single host species, either P. fluorescens or P. putida, or alternating between P. fluorescens and P. putida. Crucially, the bacterial hosts were not permitted to evolve allowing us to observe plasmid evolutionary responses in isolation.ResultsIn all treatments plasmids evolved higher conjugation rates over time. Plasmids evolved in single-host environments adapted to their host bacterial species becoming less costly, but in the case of P. fluorescens-adapted plasmids, became costlier in P. putida, suggesting an evolutionary trade-off. When evolved in the multi-host environment plasmids adapted to P. fluorescens without a higher cost in P. putida.ConclusionWhereas evolution in a single-host environment selected for host-specialist plasmids due to a fitness trade-off, this trade-off could be circumvented in the multi-host environment, leading to the evolution of host-generalist plasmids.

【 授权许可】

CC BY   
© Kottara et al. 2016