Rapid identification and typing of Yersinia pestis and other Yersinia species by matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry

BMC Microbiology

Methodology Article

Saravanan Ayyadurai¹  Didier Raoult¹  Christophe Flaudrops¹  Michel Drancourt¹ 

[1] Unité de Recherche sur les Maladies Infectieuses et Tropicales Emergentes: URMITE, UMR CNRS 6236-IRD 198, Faculté de Médecine, Université de la Méditerranée, FR48, Marseille, France;

关键词: Yersinia Enterocolitica;    rpoB Gene;    Yersinia Pestis;    Identification Score;    Yersinia Species;   

DOI  :  10.1186/1471-2180-10-285

 received in 2010-07-02, accepted in 2010-11-12,  发布年份 2010

来源: Springer

【 摘 要 】

BackgroundAccurate identification is necessary to discriminate harmless environmental Yersinia species from the food-borne pathogens Yersinia enterocolitica and Yersinia pseudotuberculosis and from the group A bioterrorism plague agent Yersinia pestis. In order to circumvent the limitations of current phenotypic and PCR-based identification methods, we aimed to assess the usefulness of matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) protein profiling for accurate and rapid identification of Yersinia species. As a first step, we built a database of 39 different Yersinia strains representing 12 different Yersinia species, including 13 Y. pestis isolates representative of the Antiqua, Medievalis and Orientalis biotypes. The organisms were deposited on the MALDI-TOF plate after appropriate ethanol-based inactivation, and a protein profile was obtained within 6 minutes for each of the Yersinia species.ResultsWhen compared with a 3,025-profile database, every Yersinia species yielded a unique protein profile and was unambiguously identified. In the second step of analysis, environmental and clinical isolates of Y. pestis (n = 2) and Y. enterocolitica (n = 11) were compared to the database and correctly identified. In particular, Y. pestis was unambiguously identified at the species level, and MALDI-TOF was able to successfully differentiate the three biotypes.ConclusionThese data indicate that MALDI-TOF can be used as a rapid and accurate first-line method for the identification of Yersinia isolates.

【 授权许可】

Unknown   
© Ayyadurai et al; licensee BioMed Central Ltd. 2010. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.