Granulocyte colony-stimulating factor increases the therapeutic efficacy of bone marrow mononuclear cell transplantation in cerebral ischemia in mice

BMC Neuroscience

Research Article

Wei Liu¹  Fang Du²  Xiang-Nan Huang²  Xue-Mei Zhang²  Dan Yang²  Rui Wang²  Chun-Jiang Yu²  Jin Fu²  Hong-Yan Hu³ 

[1] Center for Experimental Research, Second Affiliated Hospital, Harbin Medical University, 150086, Harbin, China;Department of Neurology, Second Affiliated Hospital, Harbin Medical University, 150086, Harbin, China;Department of Science Research Management, Second Affiliated Hospital, Harbin Medical University, 150086, Harbin, China;

关键词: Proliferative Cell Nuclear Antigen;    Granulocyte Colony Stimulate Factor;    Saline Group;    Focal Cerebral Ischemia;    Bone Marrow Stem Cell;   

DOI  :  10.1186/1471-2202-12-61

 received in 2011-01-27, accepted in 2011-06-24,  发布年份 2011

来源: Springer

【 摘 要 】

BackgroundBone marrow mononuclear cell (BMMC) transplantation is a promising therapy for cerebral ischemia; however, little is known if its therapeutic efficacy may be improved by co-administration of potential modulatory factors in vivo. To explore this possibility, the present study examined the effect of BMMCs and G-CSF on cell proliferation, early neuronal development and neurological function recovery in experimental cerebral ischemia relative to controls that received neither treatment.ResultIschemia/infarct area was significantly reduced in BMMCs+G-CSF group relative to animal groups treated with BMMCs only, G-CSF only or saline. Transplanted BMMCs were found to colocalize with the proliferative cell nuclear antigen (PCNA) and the immature neuronal marker doublecortin (DCX). The BMMCs+G-CSF group showed increased numerical density of cells expressing PCNA and DCX, improved performance in adhesive sticker removal test and reduced neurological function severity scores relative to other groups in a time-dependent manner.ConclusionBMMCs and G-CSF co-administration exhibits synergistic beneficial effect over time. This effect could be at least partially related to increased proliferation and differentiation of bone marrow stem cells and enhanced host brain regeneration and functional recovery. The results suggest that G-CSF can increase the therapeutic efficacy of BMMCs transplantation in an experimental mouse model of cerebral ischemia.

【 授权许可】

Unknown   
© Zhang et al; licensee BioMed Central Ltd. 2011. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.