Theobroma cacao L. pathogenesis-related gene tandem array members show diverse expression dynamics in response to pathogen colonization

BMC Genomics

Research Article

Yufan Zhang¹  Luis C. Mejia²  Edward Allen Herre³  Andrew S. Fister⁴  Mark J. Guiltinan⁵  Siela N. Maximova⁵ 

[1] Department of Electrical Engineering, Princeton University, NJ 08544, Princeton, USA;Institute for Scientific Research and High Technology Services (INDICASAT-AIP), Panama City, Panama;Smithsonian Tropical Research Institute (STRI), Box 0948, Unit 9100, DPO AA 34002-9998, Balboa, Ancon, Panama;Smithsonian Tropical Research Institute (STRI), Box 0948, Unit 9100, DPO AA 34002-9998, Balboa, Ancon, Panama;The Huck Institutes of the Life Sciences, The Pennsylvania State University, 422 Life Sciences Building, 16802, University Park, PA, USA;The Huck Institutes of the Life Sciences, The Pennsylvania State University, 422 Life Sciences Building, 16802, University Park, PA, USA;The Department of Plant Science, The Pennsylvania State University, 422 Life Sciences Building, 16802, University Park, PA, USA;

关键词: Pathogenesis-related;    PR genes;    PR proteins;    Gene duplication;    Tandem arrays;    Disease resistance;    Pathogen;    Phytophthora;    Colletotrichum;   

DOI  :  10.1186/s12864-016-2693-3

 received in 2016-01-12, accepted in 2016-05-05,  发布年份 2016

来源: Springer

【 摘 要 】

BackgroundThe pathogenesis-related (PR) group of proteins are operationally defined as polypeptides that increase in concentration in plant tissues upon contact with a pathogen. To date, 17 classes of highly divergent proteins have been described that act through multiple mechanisms of pathogen resistance. Characterizing these families in cacao, an economically important tree crop, and comparing the families to those in other species, is an important step in understanding cacao’s immune response.ResultsUsing publically available resources, all members of the 17 recognized pathogenesis-related gene families in the genome of Theobroma cacao were identified and annotated resulting in a set of ~350 members in both published cacao genomes. Approximately 50 % of these genes are organized in tandem arrays scattered throughout the genome. This feature was observed in five additional plant taxa (three dicots and two monocots), suggesting that tandem duplication has played an important role in the evolution of the PR genes in higher plants. Expression profiling captured the dynamics and complexity of PR genes expression at basal levels and after induction by two cacao pathogens (the oomycete, Phytophthora palmivora, and the fungus, Colletotrichum theobromicola), identifying specific genes within families that are more responsive to pathogen challenge. Subsequent qRT-PCR validated the induction of several PR-1, PR-3, PR-4, and PR-10 family members, with greater than 1000 fold induction detected for specific genes.ConclusionsWe describe candidate genes that are likely to be involved in cacao’s defense against Phytophthora and Colletotrichum infection and could be potentially useful for marker-assisted selection for breeding of disease resistant cacao varieties. The data presented here, along with existing cacao—omics resources, will enable targeted functional genetic screening of defense genes likely to play critical functions in cacao’s defense against its pathogens.

【 授权许可】

CC BY   
© Fister et al. 2016