Multiple mesodermal lineage differentiation of Apodemus sylvaticus embryonic stem cells in vitro

BMC Cell Biology

Research Article

Jin Niu¹  Jinli Zhang²  Zifei Wang³  Tao Wang³  Bruce T Lahn³  Xiuming Zhang³  Weihua Yu³  Weiqiang Li³  Frank Fuxiang Mao³  Andy Peng Xiang⁴  Lirong Zhang⁵  Wenyu Lai⁶ 

[1] Bellaire High School, Houston, TX, USA;Center for Stem Cell Biology and Tissue Engineering, Sun Yat-Sen University, 74# Zhongshan Road 2, 510080, Guangzhou, China;Center for Stem Cell Biology and Tissue Engineering, Sun Yat-Sen University, 74# Zhongshan Road 2, 510080, Guangzhou, China;The Key Laboratory for Stem Cells and Tissue Engineering, Ministry of Education; Key Laboratory of Stem Cells and Tissue Engineering of Guangdong Higher Education Institutes, Sun Yat-sen University, Guangzhou, China;Center for Stem Cell Biology and Tissue Engineering, Sun Yat-Sen University, 74# Zhongshan Road 2, 510080, Guangzhou, China;The Key Laboratory for Stem Cells and Tissue Engineering, Ministry of Education; Key Laboratory of Stem Cells and Tissue Engineering of Guangdong Higher Education Institutes, Sun Yat-sen University, Guangzhou, China;Department of Biochemistry, Zhongshan Medical School, Sun Yat-sen University, Guangzhou, China;Department of Pathophysiology, Guangdong College of Pharmacy, Guangzhou, China;Department of Pediatric, The Second Affiliated Hospital of Sun Yat-sen University, Yanjiang Xi Lu, 510120, Guangzhou, China;

关键词: Embryonic Stem Cell;    Retinoic Acid;    Embryoid Body;    Mouse Embryonic Stem Cell;    Embryonic Stem Cell Line;   

DOI  :  10.1186/1471-2121-11-42

 received in 2009-07-25, accepted in 2010-06-19,  发布年份 2010

来源: Springer

【 摘 要 】

BackgroundEmbryonic stem (ES) cells have attracted significant attention from researchers around the world because of their ability to undergo indefinite self-renewal and produce derivatives from the three cell lineages, which has enormous value in research and clinical applications. Until now, many ES cell lines of different mammals have been established and studied. In addition, recently, AS-ES1 cells derived from Apodemus sylvaticus were established and identified by our laboratory as a new mammalian ES cell line. Hence further research, in the application of AS-ES1 cells, is warranted.ResultsHerein we report the generation of multiple mesodermal AS-ES1 lineages via embryoid body (EB) formation by the hanging drop method and the addition of particular reagents and factors for induction at the stage of EB attachment. The AS-ES1 cells generated separately in vitro included: adipocytes, osteoblasts, chondrocytes and cardiomyocytes. Histochemical staining, immunofluorescent staining and RT-PCR were carried out to confirm the formation of multiple mesodermal lineage cells.ConclusionsThe appropriate reagents and culture milieu used in mesodermal differentiation of mouse ES cells also guide the differentiation of in vitro AS-ES1 cells into distinct mesoderm-derived cells. This study provides a better understanding of the characteristics of AS-ES1 cells, a new species ES cell line and promotes the use of Apodemus ES cells as a complement to mouse ES cells in future studies.

【 授权许可】

Unknown   
© Wang et al; licensee BioMed Central Ltd. 2010. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.