Centipede grass exerts anti-adipogenic activity through inhibition of C/EBPβ, C/EBPα, and PPARγ expression and the AKT signaling pathway in 3T3-L1 adipocytes

BMC Complementary and Alternative Medicine

Research Article

Seung Sik Lee¹  Byung Yeoup Chung¹  Suk-Nam Kang²  Gyo Moon Chu²  Young Min Song²  Hyoung Joon Park³  Gon-Sup Kim³  Min-Kwon Lee³  Yuno Song³  Jae-Hyeon Cho⁴ 

[1] Advanced Radiation Technology Institute, Korea Atomic Energy Institute, Jeongeup, South Korea;Dept. of Animal Science & Biotechnology, Gyeongnam National University of Science and Technology, Jinju, South Korea;Institute of Life Science, College of Veterinary Medicine, Gyeongsang National University, Jinju, South Korea;Institute of Life Science, College of Veterinary Medicine, Gyeongsang National University, Jinju, South Korea;Department of Anatomy and Developmental Biology, College of Veterinary Medicine, Gyeongsang National University, 900 Gajwa-dong, 660-701, Jinju-city, South Korea;

关键词: Lipid Droplet;    Adipocyte Differentiation;    Mature Adipocyte;    Adipogenic Transcription Factor;    Centipede Grass;   

DOI  :  10.1186/1472-6882-12-230

 received in 2012-05-14, accepted in 2012-10-31,  发布年份 2012

来源: Springer

【 摘 要 】

BackgroundCentipede grass (CG) originates from China and South America and is reported to contain several C-glycosyl flavones and phenolic constituents, including maysin and luteolin derivatives. This study aimed to investigate, for the first time, the antiobesity activity of CG and its potential molecular mechanism in 3T3-L1 cells.MethodsTo study the effect of CG on adipogenesis, differentiating 3T3-L1 cells were treated every day with CG at various concentrations (0–100 μg/ml) for six days. Oil-red O staining and triglyceride content assay were performed to determine the lipid accumulation in 3T3-L1 cells. The expression of mRNAs or proteins associated with adipogenesis was measured using RT-PCR and Western blotting analysis. We examined the effect of CG on level of phosphorylated Akt in 3T3-L1 cells treated with CG at various concentration s during adipocyte differentiation.ResultsDifferentiation was investigated with an Oil-red O staining assay using CG-treated 3T3-L1 adipocytes. We found that CG suppressed lipid droplet formation and adipocyte differentiation in 3T3-L1 cells in a dose-dependent manner. Treatment of the 3T3-L1 adipocytes with CG resulted in an attenuation of the expression of adipogenesis-related factors and lipid metabolic genes. The expression of C/EBPα and PPARγ, the central transcriptional regulators of adipogenesis, was decreased by the treatment with CG. The expression of genes involved in lipid metabolism, aP2 were significantly inhibited following the CG treatment. Moreover, the CG treatment down-regulated the phosphorylation levels of Akt and GSK3β.ConclusionsTaken collectively, these data indicated that CG exerts antiadipogenic activity by inhibiting the expression of C/EBPβ, C/EBPα, and PPARγ and the Akt signaling pathway in 3T3-L1 adipocytes.

【 授权许可】

Unknown   
© Park et al; licensee BioMed Central Ltd. 2012. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.