Transcriptomic and proteomic profiling of maize embryos exposed to camptothecin

BMC Plant Biology

Research Article

Carlos M Vicient¹  Nuria Sánchez-Pons¹  Nora García-Muniz¹  Sami Irar¹ 

[1] Department of Molecular Genetics, Centre for Research in Agricultural Genomics, Campus UAB, Edifici CRAG, 08193, Bellaterra (Cerdanyola del Vallés), Barcelona, Spain;

关键词: Camptothecin;    Nuclease Activity;    Embryo Axis;    Homologous Recombination Repair;    Maize Embryo;   

DOI  :  10.1186/1471-2229-11-91

 received in 2010-10-29, accepted in 2011-05-19,  发布年份 2011

来源: Springer

【 摘 要 】

BackgroundCamptothecin is a plant alkaloid that specifically binds topoisomerase I, inhibiting its activity and inducing double stranded breaks in DNA, activating the cell responses to DNA damage and, in response to severe treatments, triggering cell death.ResultsComparative transcriptomic and proteomic analyses of maize embryos that had been exposed to camptothecin were conducted. Under the conditions used in this study, camptothecin did not induce extensive degradation in the genomic DNA but induced the transcription of genes involved in DNA repair and repressed genes involved in cell division. Camptothecin also affected the accumulation of several proteins involved in the stress response and induced the activity of certain calcium-dependent nucleases. We also detected changes in the expression and accumulation of different genes and proteins involved in post-translational regulatory processes.ConclusionsThis study identified several genes and proteins that participate in DNA damage responses in plants. Some of them may be involved in general responses to stress, but others are candidate genes for specific involvement in DNA repair. Our results open a number of new avenues for researching and improving plant resistance to DNA injury.

【 授权许可】

Unknown   
© Sánchez-Pons et al; licensee BioMed Central Ltd. 2011. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.