Active chemical fractions of stem bark extract of Khaya grandifoliola C.DC and Entada africana Guill. et Perr. synergistically protect primary rat hepatocytes against paracetamol-induced damage

BMC Complementary and Alternative Medicine

Research Article

Angèle Nkouatchoua Tchana¹  Frédéric Nico Njayou¹  Paul Fewou Moundipa¹  Arnaud Fondjo Kouam¹  Brice Fredy Nemg Simo¹ 

[1] Department of Biochemistry, Laboratory of Pharmacology and Toxicology, Faculty of Science, University of Yaoundé 1, POBox 812, Yaoundé, Cameroon;

关键词: K. grandifoliola;    E. africana;    Active fractions;    Synergy;    Hepatoprotection;    Primary rat hepatocytes;    Paracetamol;   

DOI  :  10.1186/s12906-016-1169-y

 received in 2015-11-17, accepted in 2016-06-16,  发布年份 2016

来源: Springer

【 摘 要 】

BackgroundKhaya grandifoliola (Meliaceae) and Entada africana (Fabaceae) are traditionally used in Bamun (a western tribe of Cameroon) traditional medicine for the treatment of liver related diseases. In this study, the synergistic hepatoprotective effect of respective active fractions of the plants were investigated against paracetamol-induced toxicity in primary cultures of rat hepatocytes.MethodsParacetamol conferred hepatocyte toxicity, as determined by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium (MTT) assay, alanine aminotransferase (ALT), superoxide dismutase (SOD), catalase (CAT) activities, malondialdehyde (MDA) and glutathione (GSH) content assays. The crude extracts were fractionated by flash chromatography and fractions were tested for hepato-(protective and curative) activities. The most active fractions of both plants were tested individually, and in combination based on their respective half effective concentration (EC50).ResultsThe methylene chloride/methanol fractions of K. grandifoliola (75:25 v/v) (KgF25) and E. africana (90:10 v/v) (EaF10) were found to be the most hepato-protective with EC50 values of 10.30 ± 1.66 μg/ml and 13.47 ± 2.06 μg/ml respectively, comparable with that of silymarin (13.71 ± 3.87 μg/ml). These fractions and their combination significantly (P <0.05) improved cell viability, inhibited ALT leakage and MDA formation, and restored cellular CAT, SOD activities and GSH content. The combination was more effective in restoring biochemical parameters with coefficients of drugs interaction (CDI) less than 1.ConclusionThese findings demonstrate that the active fractions have synergistic action in the protection of rat hepatocytes against paracetamol-induced damage and suggest that their hepatoprotective properties may be maximized by using them in combination.

【 授权许可】

CC BY   
© The Author(s). 2016