MDA-MET-conditioned-medium augments the chemoattractant-dependent migration of MDA-MET cells towards hFOB-conditioned medium and increases collagenase activity

BMC Cancer

Research Article

Henry J. Donahue¹  Karis Chin-Quee² 

[1]Department of Biomedical Engineering, Institute of Engineering and Medicine, Virginia Commonwealth University, 601 West Main Street, 23284-3067, Richmond, VA, USA

[2]Department of Orthopaedics and Rehabilitation, Penn State College of Medicine, 17033-2391, Hershey, PA, USA

关键词: Migration;    Type 1 collagen fragment;    Collagenase;    Breast cancer;    Metastasis;    Pre-metastatic niche osteoblasts;   

DOI  :  10.1186/s12885-017-3315-4

 received in 2015-07-22, accepted in 2017-05-02,  发布年份 2017

来源: Springer

【 摘 要 】

BackgroundMetastasis of breast cancer displays site-specificity towards bone. Recently, studies have emerged indicating that primary tumors may remotely influence creation of a pre-metastatic niche. In this study, we used human fetal osteoblastic cells and MDA-MET, a metastatic and preferentially bone homing derivative of the breast cancer cell line MDA-MB-231. We examined 1) whether secreted factors from MDA-MET cells increase generation of chemoattractants by human foetal osteoblastic cells 2) whether MDA-MET cells were responsive to these chemoattractants and 3) the identity of these chemoattractants.MethodsHuman foetal osteoblastic cells were treated with conditioned medium of MDA-MET cells for 24 hours and then washed with phosphate-buffered saline. Serum-free replacement medium was conditioned by treated hFOB cells for 18 hours, before its use in in vitro quantification of MDA-MET migration. We also quantified collagen levels and collagenase activity in conditioned medium from human foetal osteoblastic cells.ResultsConditioned medium from human foetal osteoblastic cells that had been treated with MDA-MET-conditioned medium attracted more MDA-MET cells than hFOB cells pre-exposed to their own medium. This conditioned medium had increased collagenase activity. The addition of bacterial collagenase removed the ability of conditioned medium from human foetal osteoblastic cells to attract MDA-MET cells.ConclusionsOur data suggest that an increase in collagenase activity in osteoblastic cells induced by their exposure to breast cancer cell–secreted factors may increase their ability to attract breast cancer cells.

【 授权许可】

CC BY   
© The Author(s). 2017