Comparative transcriptome and proteome profiling of two Citrus sinensis cultivars during fruit development and ripening

BMC Genomics

Research Article

Jian-jun Liu¹  Bin Guan¹  Li He¹  Ke-ling Chen¹  Hong-wen Li¹  Jian He¹  Jian-hui Wang¹ 

[1] Horticulture Institute, Sichuan Academy of Agricultural Sciences, 610066, Chengdu, China;State-Sichuan Joint Engineering Laboratory of Citrus Breeding and Cultivation, 610066, Chengdu, China;The Key Laboratory of Horticultural Crops Biology and Germplasm Enhancement in Southwest Regions, Ministry of Agriculture, 610066, Chengdu, China;

关键词: Citrus sinensis;    Blood orange;    Navel orange;    Fruit quality;    Transcriptome;    Proteome;   

DOI  :  10.1186/s12864-017-4366-2

 received in 2017-09-19, accepted in 2017-12-06,  发布年份 2017

来源: Springer

【 摘 要 】

BackgroundTranscriptome and proteome analyses on fruit pulp from the blood orange ‘Zaohong’ and the navel orange ‘twenty-first century’ were performed to study Citrus sinensis quality-related molecular changes during consecutive developmental periods, including young fruit, fruit-coloring onset and fruit delayed-harvest for two months, during which fruit remained on the trees.ResultsThe time-course analysis for the fruit developmental periods indicated a complex, dynamic gene expression pattern, with the numbers of differentially expressed genes (DEGs) between the two cultivars being 119, 426 and 904 at the three continuous stages tested during fruit development and ripening. The continuous increase in total soluble solids over the course of fruit development was correlated with up-regulated sucrose phosphate synthase (SPS) transcription levels in both cultivars. Eleven differentially expressed genes between the two cultivars involved in the flavonoid pathway were significantly enriched at the onset of the fruit-coloring stage when anthocyanins were detected in blood orange alone. Among 5185 proteins, 65 up-regulated and 29 down-regulated proteins were co-expressed with their cognate mRNAs with significant transcription and protein expression levels when the fruits from the two cultivars were compared at the fruit delayed-harvest stage. Additionally, important genes participating in the γ-aminobutyric acid (GABA) shunt were activated in blood orange at two significant expression levels in the fruit delayed-harvest stage. Thus, organic acids in fruit continuously decreased during this stage.ConclusionsThis research was the first to provide a more comprehensive understanding of the differentially expressed genes involved in anthocyanin, sucrose and citrate metabolism at the transcriptome and proteome levels in C. sinensis, especially during the fruit delayed-harvest stage.

【 授权许可】

CC BY   
© The Author(s). 2017