The CRE1 carbon catabolite repressor of the fungus Trichoderma reesei: a master regulator of carbon assimilation

BMC Genomics

Research Article

Rita Linke¹  Erzsébet Fekete²  Levente Karaffa²  Erzsébet Sándor³  Antoine Margeot⁴  Thomas Portnoy⁵  Irina S Druzhinina⁶  Lukas Hartl⁶  Christian P Kubicek⁶  Bernhard Seiboth⁶  Lea Atanasova⁶  Stéphane Le Crom⁷ 

[1] Austrian Center of Industrial Biotechnology, c/o Institute of Chemical Engineering, Technische Universität Wien, Getreidemarkt 9/166, A-1060, Vienna, Austria;Department of Biochemical Engineering, Faculty of Science and Technology, University of Debrecen, P.O.Box 56, H-4010, Debrecen, Hungary;Department of Plant Protection, Faculty of Agriculture and Food Sciences and Environmental Management, University of Debrecen, Böszörményi út 138., H-4032, Debrecen, Hungary;IFP Energies nouvelles, Département Biotechnologie, 1-4 Avenue de Bois-Préau, 92852, Rueil-Malmaison Cedex, France;IFP Energies nouvelles, Département Biotechnologie, 1-4 Avenue de Bois-Préau, 92852, Rueil-Malmaison Cedex, France;École normale supérieure, Institut de Biologie de l'ENS, IBENS, Paris, F-75005 France. Inserm, U1024, Paris, F-75005 France., CNRS, UMR 8197, F-75005, Paris, France;Research Area Gene Technology and Applied Biochemistry, Institute of ChemicalEngineering, Technische Universität Wien, Getreidemarkt 9/166, A-1060, Vienna, Austria;École normale supérieure, Institut de Biologie de l'ENS, IBENS, Paris, F-75005 France. Inserm, U1024, Paris, F-75005 France., CNRS, UMR 8197, F-75005, Paris, France;

关键词: High Growth Rate;    Chemostat Culture;    Carbon Catabolite Repression;    Snf2;    Motif Pair;   

DOI  :  10.1186/1471-2164-12-269

 received in 2011-01-17, accepted in 2011-05-27,  发布年份 2011

来源: Springer

【 摘 要 】

BackgroundThe identification and characterization of the transcriptional regulatory networks governing the physiology and adaptation of microbial cells is a key step in understanding their behaviour. One such wide-domain regulatory circuit, essential to all cells, is carbon catabolite repression (CCR): it allows the cell to prefer some carbon sources, whose assimilation is of high nutritional value, over less profitable ones. In lower multicellular fungi, the C2H2 zinc finger CreA/CRE1 protein has been shown to act as the transcriptional repressor in this process. However, the complete list of its gene targets is not known.ResultsHere, we deciphered the CRE1 regulatory range in the model cellulose and hemicellulose-degrading fungus Trichoderma reesei (anamorph of Hypocrea jecorina) by profiling transcription in a wild-type and a delta-cre1 mutant strain on glucose at constant growth rates known to repress and de-repress CCR-affected genes. Analysis of genome-wide microarrays reveals 2.8% of transcripts whose expression was regulated in at least one of the four experimental conditions: 47.3% of which were repressed by CRE1, whereas 29.0% were actually induced by CRE1, and 17.2% only affected by the growth rate but CRE1 independent. Among CRE1 repressed transcripts, genes encoding unknown proteins and transport proteins were overrepresented. In addition, we found CRE1-repression of nitrogenous substances uptake, components of chromatin remodeling and the transcriptional mediator complex, as well as developmental processes.ConclusionsOur study provides the first global insight into the molecular physiological response of a multicellular fungus to carbon catabolite regulation and identifies several not yet known targets in a growth-controlled environment.

【 授权许可】

Unknown   
© Portnoy et al; licensee BioMed Central Ltd. 2011. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.