Antibiotic resistance and molecular epidemiology of Staphylococcus aureusin Nigeria

BMC Microbiology

Research Article

Kenneth Okon¹  Omotayo Oyedara²  Adebayo O Shittu³  Solayide Adesida⁴  Birgit Strommenger⁵  Ulrich Nübel⁵  Franziska Layer⁵  Wolfgang Witte⁵ 

[1] Department of Medical Microbiology, University of Maiduguri Teaching Hospital, Maiduguri, Nigeria;Department of Microbiology, Obafemi Awolowo University, Ile-Ife, Nigeria;Department of Biological Sciences, College of Science, Engineering and Technology, Osun State University, Osogbo, Nigeria;Department of Microbiology, Obafemi Awolowo University, Ile-Ife, Nigeria;Robert Koch Institute, 38855, Wernigerode, Germany;Molecular Biology and Biotechnology Division, Nigerian Institute of Medical Research, Lagos, Nigeria;Robert Koch Institute, 38855, Wernigerode, Germany;

关键词: Daptomycin;    Tigecycline;    Antibiotic Resistance Gene;    Clonal Complex;    mecA Gene;   

DOI  :  10.1186/1471-2180-11-92

 received in 2010-12-22, accepted in 2011-05-05,  发布年份 2011

来源: Springer

【 摘 要 】

BackgroundStaphylococcus aureus is an important pathogen causing a wide range of infections in the hospital and community setting. In order to have adequate information for treatment of S. aureus infections, it is crucial to understand the trends in the antibiotic-resistance patterns. In addition, the occurrence and changes in types of S. aureus, clonal identities, and their geographic spread is essential for the establishment of adequate infection control programmes. In this study, 68 S. aureus isolates obtained from clinical and non-clinical sources in Nigeria between January and April 2009 were characterized using phenotypic and molecular methods.ResultsAll the S. aureus isolates were susceptible to teicoplanin, vancomycin, phosphomycin, fusidic acid, rifampicin, daptomycin, mupirocin, linezolid and tigecycline. Sixteen percent of the isolates were resistant to oxacillin, while 55% and 72% of isolates were resistant to tetracycline and trimethoprim/sulphamethoxazole (cotrimoxazole), respectively (Table 1). There was excellent correlation between the broth microdilution assay and detection of antibiotic resistance genes by the multiplex PCR, in the determination of S. aureus resistance to erythromycin, gentamicin, methicillin and tetracycline. A total of 28 spa types were identified in the study, and the predominant spa type among the methicillin-susceptible S. aureus (MSSA) isolates was t084 (13 isolates). The t037-ST241-SCCmecIII type was the only clone identified in Maiduguri (North-East Nigeria) while in South-West Nigeria, diversity among the MRSA isolates (t451-ST8-SCCmecV; t008-ST94-SCCmecIV; t002-ST5-SCCmecV; t064-ST8-SCCmecV) was observed. The toxin genes seh and etd were detected in isolates affiliated with clonal complexes CC1, CC80 and sequence type ST25, respectively. The proportion of PVL-positive isolates among MSSA was high (40%). Most of the PVL-positive MSSA isolates were obtained from wound infections and associated with clonal complexes CC1, CC30, CC121 and with sequence type ST152.Antibiotic resistance profile of S. aureus (MSSA and MRSA) from NigeriaNumber (%) of resistant isolates among:AntibioticMSSA(n = 57)MRSA(n = 11)Total(n = 68)Penicillin49 (86)11 (100)60 (88.2)Oxacillin0 (0)11 (100)11 (16.2)Teicoplanin0 (0)0 (0)0 (0)Vancomycin0 (0)0 (0)0 (0)Gentamicin1 (1.8)9 (81.8)10 (14.7)Tetracycline27 (47.4)11 (100)38 (55.9)Ciprofloxacin12 (21.1)8 (72.7)20 (29.4)Moxifloxacin0 (0)7 (63.6)7 (10.3)Trimethoprim/sulfamethoxazole39 (68.4)10 (90.9)49 (72.1)Phosphomycin0 (0)0 (0)0 (0)Fusidic acid0 (0)0 (0)0 (0)Erythromycin2 (3.5)6 (54.5)8 (11.8)Clindamycin0 (0)6 (54.5)6 (8.8)Rifampicin0 (0)0 (0)0 (0)Daptomycin0 (0)0 (0)0 (0)Mupirocin0 (0)0 (0)0 (0)Linezolid0 (0)0 (0)0 (0)Tigecycline0 (0)0 (0)0 (0)ConclusionsThe use of phenotypic and molecular methods provided useful information on antibiotic resistance and molecular diversity of S. aureus in Nigeria. The high proportion of PVL-positive MSSA isolates affiliated to various clonal complexes and detected in all the health institutions is a major concern, both as a source of severe infections and as a potential reservoir that could lead to the emergence of PVL-positive MRSA. This study presents the first baseline information on the nature of the antibiotic resistance genes from S. aureus isolates in Nigeria. There is the need to curtail the spread and establishment of MRSA and PVL-positive MSSA clones in Nigerian health care institutions.

【 授权许可】

CC BY   
© Shittu et al; licensee BioMed Central Ltd. 2011