Quantitative trait loci associated with different polar metabolites in perennial ryegrass - providing scope for breeding towards increasing certain polar metabolites

BMC Genetics

Research Article

Christine Anne Hackett¹  Derek Stewart²  Janaki Velmurugan³  Stephen L. Byrne³  Dan Milbourne³  Susanne Barth³  Alexandre Foito⁴ 

[1] Biomathematics and Statistics Scotland, Invergowrie, DD2 5DA, Dundee, UK;Enhancing Crop Productivity and Utilisation, The James Hutton Institute, Invergowrie, DD2 5DA, Dundee, UK;Engineering and Physical Sciences, Heriot-Watt University, EH14 4AS, Edinburgh, UK;Norwegian Institute of Bioeconomy Research, Pb 115, -1431, Ås, NO, Norway;Teagasc, Crops Environment and Land Use Programme, Oak Park Research Centre, Carlow, Ireland;Teagasc, Crops Environment and Land Use Programme, Oak Park Research Centre, Carlow, Ireland;Enhancing Crop Productivity and Utilisation, The James Hutton Institute, Invergowrie, DD2 5DA, Dundee, UK;

关键词: Perennial ryegrass;    Lolium perenne;    QTL;    Polar metabolites;    Aminoacids;    Soluble carbohydrates;    Myo-inositol;    Citric acid and 2;    3-hydroxypropanoic acid;   

DOI  :  10.1186/s12863-017-0552-0

 received in 2017-03-15, accepted in 2017-09-21,  发布年份 2017

来源: Springer

【 摘 要 】

BackgroundRecent advances in the mapping of biochemical traits have been reported in Lolium perenne. Although the mapped traits, including individual sugars and fatty acids, contribute greatly towards ruminant productivity, organic acids and amino acids have been largely understudied despite their influence on the ruminal microbiome.ResultsIn this study, we used a targeted gas-chromatography mass spectrometry (GC-MS) approach to profile the levels of 25 polar metabolites from different classes (sugars, amino acids, phenolic acids, organic acids and other nitrogen-containing compounds) present in a L. perenne F2 population consisting of 325 individuals. A quantitative trait (QTL) mapping approach was applied and successfully identified QTLs regulating seven of those polar metabolites (L-serine, L-leucine, glucose, fructose, myo-inositol, citric acid and 2, 3-hydroxypropanoic acid).Two QTL mapping approaches were carried out using SNP markers on about half of the population only and an imputation approach using SNP and DArT markers on the entire population. The imputation approach confirmed the four QTLs found in the SNP-only analysis and identified a further seven QTLs.ConclusionsThese results highlight the potential of utilising molecular assisted breeding in perennial ryegrass to modulate a range of biochemical quality traits with downstream effects in livestock productivity and ruminal digestion.

【 授权许可】

CC BY   
© The Author(s). 2017

【 图 表 】

12864_2017_4363_Article_IEq5.gif

12864_2017_4020_Article_IEq26.gif

12864_2015_2137_Article_IEq11.gif

12864_2017_4020_Article_IEq25.gif

12864_2017_3487_Article_IEq69.gif

12864_2016_2816_Article_IEq7.gif

12864_2017_3487_Article_IEq68.gif

12864_2017_3733_Article_IEq73.gif

12864_2017_4020_Article_IEq22.gif

12864_2015_2252_Article_IEq2.gif

12864_2016_3440_Article_IEq14.gif

12864_2016_3169_Article_IEq16.gif

12864_2017_3492_Article_IEq22.gif

12864_2017_4320_Article_IEq2.gif

12864_2016_3169_Article_IEq15.gif

12864_2015_2137_Article_IEq5.gif

12864_2017_4133_Article_IEq38.gif

12864_2017_3655_Article_IEq6.gif

12864_2017_3605_Article_IEq18.gif

12864_2017_4190_Article_IEq10.gif