Molecular Characterization of Giardia duodenalis in Children in Kenya

BMC Infectious Diseases

Research Article

C. Mbae¹  F. Guleid¹  E. Mulinge¹  S. Kariuki¹  J. Wainaina²  A. Waruru³  Z. K. Njiru⁴ 

[1] Centre for Microbiological Research, KEMRI, P.O Box 19464–00202, Nairobi, Kenya;International Livestock Research Institute, P.O Box 30709, Naivasha Rd, Nairobi, Kenya;Kenya Medical Research Institute, P. O Box 58540–00200, Nairobi, Kenya;Murdoch University, School of Health Professions, Peel Campus, 6210, Mandurah, WA, Australia;

关键词: Giardia;    Informal settlements;    Children;    Kenya;    Genotyping;    Subtyping;   

DOI  :  10.1186/s12879-016-1436-z

 received in 2015-09-04, accepted in 2016-02-16,  发布年份 2016

来源: Springer

【 摘 要 】

BackgroundGiardia duodenalis is an important intestinal protozoan in humans worldwide with high infection rates occurring in densely populated and low resource settings. The parasite has been recorded to cause diarrhea in children. This study was carried out to identify G. duodenalis assemblages and sub-assemblages in children presenting with diarrhea in Kenya.MethodsA total of 2112 faecal samples were collected from children aged ≤5 years and screened for the presence of Giardia cysts using microscopy. A total of 96 (4.5 %) samples were identified as Giardia positive samples and were genotyped using glutamate dehydrogenase (gdh), triose phosphate isomerase (tpi) and β-giardin loci.ResultsThe three markers successfully genotyped 72 isolates and grouped 2 (1.4) isolates as Assemblage A, 64 (88.9) as Assemblage B and 7 (9.7 %) consisted of mixed infections with assemblage A and B. A further analysis of 50 isolates using GDH Polymerase Chain Reaction and Restriction Fragment Length Polymorphism (PCR-RFLP) categorized 2 assemblage A isolates as sub-assemblage AII while 6 and 14 assemblage B isolates were categorized into sub-assemblage BIII and BIV respectively. A mixed infection with sub-assemblage BIII and BIV was recorded in 28 isolates. Over half (55.6 %) of Giardia infections were recorded among the children between 13 to 48 months old.ConclusionThis paper reports the first data on the assemblages and sub-assemblages of Giardia duodenalis in children representing with diarrhea in Kenya.

【 授权许可】

CC BY   
© Mbae et al. 2016