Identification of potential candidate genes for hypertensive nephropathy based on gene expression profile

BMC Nephrology

Research Article

Ye Feng¹  Guohua Wang²  Zhi Chen³  Hao Wu³ 

[1] Department of Gastrointestinal Colorectal and Anal Surgery, China-Japan Union Hospital of Jilin University, No.126 Xiantai Avenue, 130033, Jilin, China;Department of Neonatology, First Hospital of Jilin University, 130021, Jilin, China;Department of Nephrology, First Hospital of Jilin University, 130021, Jilin, China;

关键词: Hypertensive nephropathy;    Differentially expressed gene;    Pathway;    Network;    microRNA;   

DOI  :  10.1186/s12882-016-0366-8

 received in 2015-11-09, accepted in 2016-10-11,  发布年份 2016

来源: Springer

【 摘 要 】

BackgroundThis study was aimed to explore the molecular mechanisms of hypertensive nephropathy (HTN).MethodsGene expression profile of GSE37460, which based on 27 healthy living donor samples (HTN group) and 15 hypertensive nephropathy samples (control group), were downloaded from Gene Expression Omnibus (GEO) database. The differentially expressed genes (DEGs) between two groups were identified. STRING database was used to reveal protein-protein interaction (PPI) network of DEGs, followed by the functional enrichment analysis of the PPI network. Additionally, miRNA-DEG regulatory network was constructed to reveal the validated miRNAs targeting the DEGs.ResultsIn total, 51 up-regulated genes and 140 down-regulated genes were obtained. In the PPI network, cytochrome P450 3A4 (CYP3A4) and angiotensin II receptor type 1 (AGTR1) had a higher degree, and CYP3A4 interacted with CYP4A11. The DEGs in the network were significantly enriched in drug metabolism, focal adhesion and arachidonic acid metabolism. Furthermore, in the miRNA-DEG regulatory network, hsa-miR-335-5p and hsa-miR-26b-5p were the two most outstanding miRNAs. AGTR1, CYP3A4 and CYP4A11 were predicted to be regulated by hsa-miR-26b-5p.ConclusionThe DEGs, such as AGTR1, CYP3A4 and CYP4A11 may play critical roles in the development of HTN likely via the regulation by hsa-miR-26b-5p and taking part in some pathways.

【 授权许可】

CC BY   
© The Author(s). 2016