Glucosamine sulfate suppresses the expression of matrix metalloproteinase-3 in osteosarcoma cells in vitro

BMC Complementary and Alternative Medicine

Research Article

Christian Suren¹  Florian Pohlig¹  Philipp Mayer-Kuckuk¹  Ulrich Lenze¹  Rüdiger von Eisenhart-Rothe¹  Jörg Ulrich¹  Johannes Schauwecker¹  Norbert Harrasser¹  Heinrich M. L. Mühlhofer¹ 

[1] Department of Orthopedic Surgery, Klinikum rechts der Isar, Technical University Munich, Ismaninger Str. 22, 81675, Munich, Germany;

关键词: Glucosamine sulfate;    Neutraceutical;    Dietary supplement;    Osteosarcoma;    Matrix metalloproteinase;    MMP-3;    MMP-9;   

DOI  :  10.1186/s12906-016-1315-6

 received in 2016-04-06, accepted in 2016-08-23,  发布年份 2016

来源: Springer

【 摘 要 】

BackgroundGlucosamine, a common dietary supplement, has a possible anti-sarcoma effect. However, an understanding of the underlying mechanism of such an effect is limited. For this study we hypothesized that glucosamine suppresses the basal level of matrix metalloproteinase expression in human osteosarcoma cell lines.MethodsWe examined the osteosarcoma cell lines, MG-63 and SaOS-2. Cells were exposed to 0, 10, 50 and 100 μg/ml glucosamine sulfate for 48 h and treatment toxicity was determined through measurement of cell viability and proliferation. Relative gene expression of matrix metalloproteinase (MMP)-2, -3 and -9 was quantified by real-time polymerase chain reaction. Protein levels of MMP-2 and -9 were assessed by ELISA.ResultsAdministration of 10, 50 or 100 μg/ml glucosamine sulfate had no effect on the cell viability of MG-63 and SaOS-2 cells. A significant reduction of MMP expression in both cell lines was observed only for MMP-3, while a decrease in MMP-9 was seen in SaOS-2 cells. The expression of MMP-2 was not significantly affected in either cell line. Protein level of MMP-3 was reduced in both cell lines upon stimulation with 10 μg/ml glucosamine sulfate whereas for MMP-9 a decrease could only be observed in SaOS-2 cells.ConclusionIn this study, we found a pronounced suppressive effect of glucosamine sulfate particularly on MMP-3 and also MMP-9 mRNA and protein levels in osteosarcoma cell lines in vitro. The data warrants further investigations into the potential anti-tumor efficacy of glucosamine sulfate in osteosarcoma.

【 授权许可】

CC BY   
© The Author(s). 2016