Metabolic alteration of urinary steroids in pre- and post-menopausal women, and men with papillary thyroid carcinoma

BMC Cancer

Research Article

Eun Jig Lee¹  Ju-Yeon Moon²  Bong Chul Chung²  Man Ho Choi²  Sung-Hee Cho² 

[1] Department of Internal Medicine, Yonsei University College of Medicine, 120-752, Seoul, South Korea;Future Convergence Research Division, Korea Institute of Science and Technology, 136-791, Seoul, South Korea;

关键词: Steroids;    Thyroid cancer;    Menopause;    Gender difference;    GC-MS;   

DOI  :  10.1186/1471-2407-11-342

 received in 2011-03-07, accepted in 2011-08-08,  发布年份 2011

来源: Springer

【 摘 要 】

BackgroundTo evaluate the metabolic changes in urinary steroids in pre- and post-menopausal women and men with papillary thyroid carcinoma (PTC).MethodsQuantitative steroid profiling combined with gas chromatography-mass spectrometry was used to measure the urinary concentrations of 84 steroids in both pre- (n = 21, age: 36.95 ± 7.19 yr) and post-menopausal female (n = 19, age: 52.79 ± 7.66 yr), and male (n = 16, age: 41.88 ± 8.48 yr) patients with PTC. After comparing the quantitative data of the patients with their corresponding controls (pre-menopause women: n = 24, age: 33.21 ± 10.48 yr, post-menopause women: n = 16, age: 49.67 ± 8.94 yr, male: n = 20, age: 42.75 ± 4.22 yr), the levels of steroids in the patients were normalized to the mean concentration of the controls to exclude gender and menopausal variations.ResultsMany urinary steroids were up-regulated in all PTC patients compared to the controls. Among them, the levels of three active androgens, androstenedione, androstenediol and 16α-hydroxy DHEA, were significantly higher in the pre-menopausal women and men with PTC. The corticoid levels were increased slightly in the PTC men, while progestins were not altered in the post-menopausal PTC women. Estrogens were up-regulated in all PTC patients but 2-hydroxyestrone and 2-hydroxy-17β-estradiol were remarkably changed in both pre-menopausal women and men with PTC. For both menopausal and gender differences, the 2-hydroxylation, 4-hydroxylation, 2-methoxylation, and 4-methoxylation of estrogens and 16α-hydroxylation of DHEA were differentiated between pre- and post-menopausal PTC women (P < 0.001). In particular, the metabolic ratio of 2-hydroxyestrone to 2-hydroxy-17β-estradiol, which could reveal the enzyme activity of 17β-hydroxysteroid dehydrogenase, showed gender differences in PTC patients (P < 1 × 10-7).ConclusionsThese results are expected be helpful for better understanding the pathogenic differences in PTC according to gender and menopausal conditions.

【 授权许可】

Unknown   
© Choi et al; licensee BioMed Central Ltd. 2011. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.