Pharmacological screening of Monotheca buxifolia (Falc.) A. DC. for antinociceptive, anti-inflammatory and antipyretic activities

BMC Complementary and Alternative Medicine

Research Article

Ajmal Khan¹  Umar Farooq¹  Ibrahim Javed²  Jamshaid Ali Khan³  Peer Abdul Hannan³  Faisal Shakeel³  Muhammad Shahid³  Irfan Ullah⁴  Achyut Adhikari⁵ 

[1] Department of Chemistry, COMSATS Institute of Information Technology, Abbottabad, Pakistan;Department of Chemistry, SBA School of Science and Engineering, Lahore University of Management Sciences, Lahore, Pakistan;Department of Pharmacy, University of Peshawar, 25120, Peshawar, Pakistan;Department of Pharmacy, University of Peshawar, 25120, Peshawar, Pakistan;Department of Pharmacy, Abasyn University, Peshawar, Pakistan;HEJ Research Institute of Chemistry, International Centre for Chemical and Biological Sciences, University of Karachi, Karachi, Pakistan;

关键词: Sapotaceae;    Oleanolic acid;    Isoquercetin;    Pain;    Inflammation;    Pyrexia;   

DOI  :  10.1186/s12906-016-1257-z

 received in 2016-02-28, accepted in 2016-08-02,  发布年份 2016

来源: Springer

【 摘 要 】

BackgroundMedicinal plants have proven their importance as a valuable source of molecules with therapeutic potential. Monotheca buxifolia (Falc.) A. DC. (family: Sapotaceae) is traditionally used as a hematinic, laxative, digestive, anthelmintic, antipyretic, and in the treatment of gastro-urinary disorders. To provide scientific evidence for its folkloric use, the present study investigated Monotheca buxifolia fruit hydro-ethanolic extract (MBHE) for its prospective antinociceptive, anti-inflammatory and antipyretic activities. MBHE was eluted through column chromatography to isolate the bioactive secondary metabolites which may probably involve in its beneficial properties.MethodsThe phytochemical constituents in MBHE was elucidated using UV, IR, 1H-NMR, 13C NMR, 2D-NMR spectra in combination with EIMS and FAB-MS spectrometric techniques and comparison with literature data of related compounds. The antinociceptive activity of MBHE was evaluated in the acetic acid induced abdominal constriction assay; the anti-inflammatory potential was assessed in the carrageenan induced paw edema, while the antipyretic effect was tested against brewer’s yeast induced pyrexia in BALB/c mice at doses of 50, 100 and 150 mg/kg.ResultsElution of MBHE along with various characterization techniques led to the isolation of oleanolic acid and isoquercetin. Significant attenuation of chemical induced nociception was observed with MBHE at tested doses of 50 mg/kg (P < 0.01, 68.87 %), 100 mg/kg (P < 0.01, 68.87 %) and 150 mg/kg (P < 0.001, 83.02 %). During a duration of 1–5 h in the carrageenan induced paw edema assay, significant ameliorative effect (P < 0.01, P < 0.001) was demonstrated by MBHE at 50 mg/kg (22.94–20 %), 100 mg/kg (33.23–21.13 %) and 150 mg/kg (38.23–25 %). MBHE also significantly alleviated the brewer’s yeast induced pyrexic response when tested at doses of 50 mg/kg (P < 0.05 in 2nd h), 100 mg/kg (P < 0.05, P < 0.01 and P < 0.001 in 1–5 h) and 150 mg/kg (P < 0.01 and P < 0.001 in 1–5 h).ConclusionThese findings suggest that Monotheca buxifolia possess pain, inflammation and pyrexia ameliorating properties, probably mediated by the presence of oleanolic acid and isoquercetin contents, though the involvement of other important phytochemicals constituents cannot be ignored.

【 授权许可】

CC BY   
© The Author(s). 2016