Reaction of rat connective tissue to mineral trioxide aggregate and diaket

BMC Oral Health

Research Article

Wael M Al-Omari¹  Huda M Hammad²  Maisoun S Abu-Zaghlan³ 

[1] Associate Professor, Department of Conservative Dentistry, Faculty of Dentistry, Jordan University of Science and Technology, Irbid, Jordan;Associate Professor, Department of Oral Medicine and Surgery, Faculty of Dentistry, Jordan University of Science and Technology, Irbid, Jordan;Endodontist, Private sector, Amman, Jordan;

关键词: Median Score;    Calcium Hydroxide;    Mineral Trioxide Aggregate;    Dentine Tube;    Fibroblastic Activity;   

DOI  :  10.1186/1472-6831-11-17

 received in 2010-11-05, accepted in 2011-05-13,  发布年份 2011

来源: Springer

【 摘 要 】

BackgroundThe aim of this study was to compare the reaction of rat connective tissue to two root-end filling materials: white Mineral Trioxide Aggregate (WMTA) and Diaket.MethodsEach of the materials was placed in dentine tubes and implanted subcutaneously in the dorsal connective tissue of 21 Wistar albino rats. Tissue biopsies were collected 7, 30, and 60 days after the implantation procedure. The specimens were processed and stained with hematoxylin and eosin and examined microscopically. After determining inflammatory cell numbers in sections from each specimen, inflammatory reaction scores were defined as follows: 0; no or few inflammatory cells (no reaction), 1; less than 25 cells (mild reaction), 2; 25 to 125 cells, (moderate reaction), and 3; 125 or more cells (severe reaction). Statistical analysis was performed using the Kruskal-Wallis and Mann-Whitney tests.ResultsThere were statistically significant differences in the median inflammatory cell numbers throughout the three test periods, with the most severe degree of inflammation observed at the one-week period. Few cases of necrosis were observed with WMTA. Diaket exhibited the most severe degree of inflammation and necrosis. After 30 days, both materials provoked moderate inflammatory reaction. The eight-week period showed the least severe degree of inflammation in all groups.ConclusionsIt was concluded that WMTA exhibits a more favourable tissue response compared with Diaket which induced more severe inflammatory reaction than WMTA and the control.

【 授权许可】

CC BY   
© Al-Omari et al; licensee BioMed Central Ltd. 2011