Molecular analysis of the benthos microbial community in Zavarzin thermal spring (Uzon Caldera, Kamchatka, Russia)

BMC Genomics

Research

Oksana P Taran¹  Sergey E Peltek²  Irina A Meshcheryakova²  Timofey V Ivanisenko²  Alla V Bryanskaya²  Tatiana K Malup²  Alexey S Rozanov²  Vladimir A Ivanisenko³  Nikolay A Kolchanov³  Elena V Lazareva⁴  Sergey M Zhmodik⁵ 

[1] Boreskov Institute of Catalysis SB RAS, 630090, Novosibirsk, Russia;Institute of Cytology & Genetics SB RAS, 630090, Novosibirsk, Russia;Institute of Cytology & Genetics SB RAS, 630090, Novosibirsk, Russia;Novosibirsk State University, 630090, Novosibirsk, Russia;V S Sobolev Institute of Geology and Mineralogy SB RAS, 630090, Novosibirsk, Russia;V S Sobolev Institute of Geology and Mineralogy SB RAS, 630090, Novosibirsk, Russia;Novosibirsk State University, 630090, Novosibirsk, Russia;

关键词: Microbiology;    metagenome;    Zavarzin thermal spring;    Uzon Caldera;    Kamchatka;   

DOI  :  10.1186/1471-2164-15-S12-S12

来源: Springer

【 摘 要 】

BackgroundGeothermal areas are of great interest for the study of microbial communities. The results of such investigations can be used in a variety of fields (ecology, microbiology, medicine) to answer fundamental questions, as well as those with practical benefits. Uzon caldera is located in the Uzon-Geyser depression that is situated in the centre of the Karym-Semyachin region of the East Kamchatka graben-synclinorium. The microbial communities of Zavarzin spring are well studied; however, its benthic microbial mat has not been previously described.ResultsPyrosequencing of the V3 region of the 16S rRNA gene was used to study the benthic microbial community of the Zavarzin thermal spring (Uzon Caldera, Kamchatka). The community is dominated by bacteria (>95% of all sequences), including thermophilic, chemoorganotrophic Caldiserica (33.0%) and Dictyoglomi (24.8%). The benthic community and the previously examined planktonic community of Zavarzin spring have qualitatively similar, but quantitatively different, compositions.ConclusionsIn this study, we performed a metagenomic analysis of the benthic microbial mat of Zavarzin spring. We compared this benthic community to microbial communities found in the water and of an integral probe consisting of water and bottom sediments. Various phylogenetic groups of microorganisms, including potentially new ones, represent the full-fledged trophic system of Zavarzin. A thorough geochemical study of the spring was performed.

【 授权许可】

Unknown   
© Rozanov et al.; licensee BioMed Central Ltd. 2014. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.