Gamma-tocotrienol treatment increased peroxiredoxin-4 expression in HepG2 liver cancer cell line

BMC Complementary and Alternative Medicine

Research Article

Farahani Abdul Rahman Sazli¹  Zakiah Jubri¹  Wan Zurinah Wan Ngah¹  Mariati Abdul Rahman²  Saiful Anuar Karsani³  Abdul Gapor Md Top⁴ 

[1] Department of Biochemistry, Universiti Kebangsaan Malaysia Medical Centre, Kuala Lumpur, Malaysia;Department of Clinical Oral Biology, Faculty of Dentistry, Universiti Kebangsaan Malaysia, Kuala Lumpur, Malaysia;Institute of Biological Sciences, Faculty of Science, University of Malaya and University of Malaya Centre for Proteomics Research (UMCPR), Kuala Lumpur, Malaysia;Malaysian Palm Oil Board, Bangi, Selangor, Malaysia;

关键词: Proteomics;    Gamma-tocotrienol;    HepG2 cells;    Peroxiredoxin-4;   

DOI  :  10.1186/s12906-015-0590-y

 received in 2013-09-20, accepted in 2015-02-25,  发布年份 2015

来源: Springer

【 摘 要 】

BackgroundTo determine the antiproliferative effect of gamma-tocotrienol (GTT) treatment on differential protein expression in HepG2 cells.MethodsHepG2 cells were treated with 70 μM GTT for 48 hours and differentially expressed protein spots were determined by two-dimensional electrophoresis (2DE), identified by MALDI-TOF mass spectrometer (MS) and validated by quantitative real-time polymerase chain reaction (qRT-PCR).ResultsGTT treatment on HepG2 cells showed a total of five differentially expressed proteins when compared to their respective untreated cells where three proteins were down-regulated and two proteins were up-regulated. One of these upregulated proteins was identified as peroxiredoxin-4 (Prx4). Validation by qRT-PCR however showed decreased expression of Prx4 mRNA in HepG2 cells following GTT treatment.ConclusionsGTT might directly influence the expression dynamics of peroxiredoxin-4 to control proliferation in liver cancer.

【 授权许可】

Unknown   
© Abdul Rahman Sazli et al.; licensee BioMed Central. 2015. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.