Expression profiling of prospero in the Drosophila larval chemosensory organ: Between growth and outgrowth

BMC Genomics

Research Article

Rémi Houlgatte¹  Mahatsangy Raharijaona¹  Laure Guenin²  Fawzia Baba-Aissa³ 

[1] INSERM, U915, F-44000, Nantes, France;Université de Nantes, l'Institut du Thorax, F-44000, Nantes, France;Institut Pasteur, Pathogénomique Mycobactérienne Intégrée, 25, Rue du Dr. Roux, 75724, Paris Cedex 15, France;Université de Bourgogne, Facultés des Sciences, Unité Mixte de Recherche 5548 Associée au Centre National de la Recherche Scientifique, 6, Bd Gabriel, 21 000, Dijon, France;Université de Bourgogne, Facultés des Sciences, Unité Mixte de Recherche 5548 Associée au Centre National de la Recherche Scientifique, 6, Bd Gabriel, 21 000, Dijon, France;

关键词: Gene Ontology;    Neurite Outgrowth;    Central Nervous System Tissue;    Position Weight Matrix;    Ventral Organ;   

DOI  :  10.1186/1471-2164-11-47

 received in 2009-03-17, accepted in 2010-01-19,  发布年份 2010

来源: Springer

【 摘 要 】

BackgroundThe antenno-maxilary complex (AMC) forms the chemosensory system of the Drosophila larva and is involved in gustatory and olfactory perception. We have previously shown that a mutant allele of the homeodomain transcription factor Prospero (prosVoila1, V1), presents several developmental defects including abnormal growth and altered taste responses. In addition, many neural tracts connecting the AMC to the central nervous system (CNS) were affected. Our earlier reports on larval AMC did not argue in favour of a role of pros in cell fate decision, but strongly suggested that pros could be involved in the control of other aspect of neuronal development. In order to identify these functions, we used microarray analysis of larval AMC and CNS tissue isolated from the wild type, and three other previously characterised prospero alleles, including the V1 mutant, considered as a null allele for the AMC.ResultsA total of 17 samples were first analysed with hierarchical clustering. To determine those genes affected by loss of pros function, we calculated a discriminating score reflecting the differential expression between V1 mutant and other pros alleles. We identified a total of 64 genes in the AMC. Additional manual annotation using all the computed information on the attributed role of these genes in the Drosophila larvae nervous system, enabled us to identify one functional category of potential Prospero target genes known to be involved in neurite outgrowth, synaptic transmission and more specifically in neuronal connectivity remodelling. The second category of genes found to be differentially expressed between the null mutant AMC and the other alleles concerned the development of the sensory organs and more particularly the larval olfactory system. Surprisingly, a third category emerged from our analyses and suggests an association of pros with the genes that regulate autophagy, growth and insulin pathways. Interestingly, EGFR and Notch pathways were represented in all of these three functional categories. We now propose that Pros could perform all of these different functions through the modulation of these two antagonistic and synergic pathways.ConclusionsThe current data contribute to the clarification of the prospero function in the larval AMC and show that pros regulates different function in larvae as compared to those controlled by this gene in embryos. In the future, the possible mechanism by which Pros could achieve its function in the AMC will be explored in detail.

【 授权许可】

Unknown   
© Guenin et al; licensee BioMed Central Ltd. 2010. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.