The expression of 11β-hydroxysteroid dehydrogenase type 1 is increased in experimental periodontitis in rats

BMC Oral Health

Research Article

Hirofumi Sawai¹  Takaya Nakata²  Makoto Umeda³  Hiroaki Masuzaki⁴ 

[1] Department of Internal Medicine, Osaka Dental University, Hirakata, Osaka, Japan;Department of Periodontology, Graduate School of Dentistry, Osaka Dental University, Hirakata, Osaka, Japan;Department of Periodontology, Osaka Dental University, Hirakata, Osaka, Japan;Division of Endocrinology, Diabetes and Metabolism, Hematology, Rheumatology, Graduate School of Internal Medicine, University of the Ryukyus, Nakagami-gun, Okinawa, Japan;

关键词: 11β-Hydroxysteroid dehydrogenase type 1;    Glucocorticoids;    Chronic periodontitis;    Experimental periodontitis;    Inflammation;   

DOI  :  10.1186/s12903-016-0303-z

 received in 2016-07-05, accepted in 2016-09-22,  发布年份 2016

来源: Springer

【 摘 要 】

BackgroundThe involvement of 11β-hydroxysteroid dehydrogenase type 1 (11β-HSD1), which converts inactive glucocorticoids into active glucocorticoids intracellularly, in metabolic diseases and chronic inflammatory diseases has been elucidated. We recently reported that an increase in 11β-HSD1 expression was associated with chronic periodontitis in humans irrespective of obesity. To further clarify the role of 11β-HSD1 in chronic periodontitis, the expression of 11β-HSD1 was investigated in experimental periodontitis model in rats.MethodsExperimental periodontitis was induced by silk ligature of left maxillary second molars of 7-week-old male Wistar rats, and periodontal tissues were collected at day 3. The expression of 11β-HSD1, 11β-HSD2, and TNFα mRNA was examined using real time reverse transcription-polymerase chain reaction. The expression of TNFα was used as an indicator of inflammation. Thus, the rats in which the levels of TNFα mRNA were increased in the ligature-induced periodontitis compared with the control were analysed.ResultsThe findings demonstrated that the expression of 11β-HSD1 mRNA was significantly increased in experimental periodontitis compared with the control. The increase in the levels of 11β-HSD1 mRNA in the ligature-induced periodontitis compared with the control was positively correlated with that of TNFα mRNA. On the other hand, the expression of 11β-HSD2 mRNA, which inactivates glucocorticoids, was slightly decreased in experimental periodontitis. Therefore, the ratio of 11β-HSD1 versus 11β-HSD2 mRNA was significantly higher in experimental periodontitis than in the control.ConclusionsThese results suggest that the increased expression of 11β-HSD1, which would result in the increased levels of intracellular glucocorticoids, may play a role in the pathophysiology of experimental periodontitis.

【 授权许可】

CC BY   
© The Author(s). 2016