Serotype and genotype analysis of dengue virus by sequencing followed by phylogenetic analysis using samples from three mini outbreaks-2007-2009 in Pakistan

BMC Microbiology

Research Article

Zarfishan Tahir¹  Obaid Ullah¹  Mohammad A Bajwa²  Muhammad Q Zia²  Saira Munir³  Sadaf Badar³  Liaqat Ali³  Muhammad Ali³  Abrar Hussain³  Sadia Butt³  Madiha Akram³  Muhammad Idrees³  Mahrukh Naudhani³  Sana Saleem³  Bushra Khubaib³  Samia Afzal³  Zareen Fatima³  Bisma Rauff³  Mahwish Aftab³ 

[1] Bacteriologist Laboratory, Institute of Public Health, 6-Birdwood Road, Lahore, Pakistan;Department of Gastroenterology, Sheikh Zayed Medical Complex, Lahore, Pakistan;Division of Molecular Virology, CEMB University of the Punjab, 87-West Canal Bank Road, 53700, Thokar Niaz Baig, Lahore, Pakistan;

关键词: West Nile Virus;    Dengue Virus;    Nest Polymerase Chain Reaction;    Dengue Hemorrhagic Fever;    Dengue Infection;   

DOI  :  10.1186/1471-2180-11-200

 received in 2011-02-01, accepted in 2011-09-10,  发布年份 2011

来源: Springer

【 摘 要 】

BackgroundSince the first reported outbreak of dengue hemorrhagic fever in Pakistan, several mini outbreaks have erupted in the region. Dengue virus serotype 3 (DEN-3) was first documented in 2005 outbreak in Karachi. Reports show that serotype 3 is prevalent in Lahore since 2008. Serotype 2 (DEN-2) is the major circulating serotype in Pakistan as it is documented since 1994. We have conducted a detailed study of three outbreaks of dengue virus infection that occurred in years 2007, 2008 and 2009 in Lahore by using molecular techniques such as PCR and nucleotide sequencing of the C-prM gene junction of Dengue virus.ResultsThrough the analysis of 114 serum samples collected over the period of three years (2007-2009), total 20 patients were found to be infected with dengue virus. In year 2007, four were positive for serotype 2 and one sample was positive for serotype DEN-3. In 2008, five samples had concurrent infection with serotypes DEN-2 and DEN-3 while three samples were infected only with serotype DEN-2. In year 2009, one sample had concurrent infection with serotypes DEN-2 and DEN-3 while six were positive for serotype DEN-2 only.ConclusionsOur study showed that serotype DEN-2 was dominant in positive samples of dengue virus infection collected during the period of three years (2007-2009). The other serotype present was serotype DEN-3. Genotypes of serotype DEN-2 and serotype DEN-3 were subtype IV and subtype III, respectively.

【 授权许可】

Unknown   
© Fatima et al; licensee BioMed Central Ltd. 2011. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.