BMC Biotechnology | |
Evaluating the expression profile and stability of different UCOE containing vector combinations in mAb-producing CHO cells | |
Research Article | |
Fereidoun Mahboudi1  Fatemeh Davami1  Fatemeh Nematpour1  Samira Ahmadi1  Behrouz Vaziri1  Saedeh Ebadat1  Vahid Khalaj1  Maryam Ahmadi2  | |
[1] Biotechnology Research Center, Pasteur Institute of Iran, 1316943551, Tehran, Iran;Biotechnology Research Center, Pasteur Institute of Iran, 1316943551, Tehran, Iran;Departments of Medical Biotechnology, Semnan University of Medical Sciences, 3519899951, Semnan, Iran; | |
关键词: Cell line development; Chinese hamster ovary (CHO); Monoclonal antibody (mAb); Ubiquitous chromatin opening elements (UCOE); | |
DOI : 10.1186/s12896-017-0330-0 | |
received in 2016-07-27, accepted in 2017-02-03, 发布年份 2017 | |
来源: Springer | |
【 摘 要 】
BackgroundAs the demand for monoclonal antibodies (mAb) increases, more efficient expression methods are required for their manufacturing process. Transcriptional gene silencing is a common phenomenon in recombinant cell lines which leads to expression reduction and instability. There are reports on improved antibody expression in ubiquitous chromatin opening element (UCOE) containing both heavy and light chain gene constructs. Here we investigate the impact of having these elements as part of the light chain, heavy chain or both genes during cell line development. In this regard, non-UCOE and UCOE vectors were constructed and stable Chinese hamster ovary (CHO) cell pools were generated by different vector combinations.ResultsExpression analysis revealed that all UCOE cell pools had higher antibody yields compared to non-UCOE cells, Moreover the most optimal expression was obtained by cells containing just the UCOE on heavy chain. In terms of stability, it was shown that the high level of expression was kept consistence for more than four months in these cells whereas the expression titers were reduced in the other UCOE pools.ConclusionsIn conclusion, UCOE significantly enhanced the level and stability of antibody expression and the use of this element with heavy chain provided more stable cell lines with higher production level.
【 授权许可】
CC BY
© The Author(s). 2017
【 预 览 】
Files | Size | Format | View |
---|---|---|---|
RO202311096537483ZK.pdf | 1295KB | download |
【 参考文献 】
- [1]
- [2]
- [3]
- [4]
- [5]
- [6]
- [7]
- [8]
- [9]
- [10]
- [11]
- [12]
- [13]
- [14]
- [15]
- [16]
- [17]
- [18]
- [19]
- [20]
- [21]
- [22]
- [23]
- [24]
- [25]
- [26]
- [27]
- [28]
- [29]
- [30]
- [31]
- [32]