Identification of novel mitosis regulators through data mining with human centromere/kinetochore proteins as group queries

BMC Cell Biology

Research Article

Shermeen Sufi¹  Peter Oladimeji¹  Song-Tao Liu¹  Aaron R Tipton¹  Kexi Wang¹  Zhidong Gu² 

[1] Department of Biological Sciences, University of Toledo, 43606, Toledo, OH, USA;Department of Biological Sciences, University of Toledo, 43606, Toledo, OH, USA;Ruijin Hospital, 200025, Shanghai, China;

关键词: Centromere;    Kinetochore;    Centrosome;    Data mining;    Protein-protein interaction;    Co-expression;   

DOI  :  10.1186/1471-2121-13-15

 received in 2012-02-13, accepted in 2012-06-19,  发布年份 2012

来源: Springer

【 摘 要 】

BackgroundProteins functioning in the same biological pathway tend to be transcriptionally co-regulated or form protein-protein interactions (PPI). Multiple spatially and temporally regulated events are coordinated during mitosis to achieve faithful chromosome segregation. The molecular players participating in mitosis regulation are still being unravelled experimentally or using in silico methods.ResultsAn extensive literature review has led to a compilation of 196 human centromere/kinetochore proteins, all with experimental evidence supporting the subcellular localization. Sixty-four were designated as “core” centromere/kinetochore components based on peak expression and/or well-characterized functions during mitosis. By interrogating and integrating online resources, we have mined for genes/proteins that display transcriptional co-expression or PPI with the core centromere/kinetochore components. Top-ranked hubs in either co-expression or PPI network are not only enriched with known mitosis regulators, but also contain candidates whose mitotic functions are not yet established. Experimental validation found that KIAA1377 is a novel centrosomal protein that also associates with microtubules and midbody; while TRIP13 is a novel kinetochore protein and directly interacts with mitotic checkpoint silencing protein p31comet.ConclusionsTranscriptional co-expression and PPI network analyses with known human centromere/kinetochore proteins as a query group help identify novel potential mitosis regulators.

【 授权许可】

Unknown   
© Tipton et al.; licensee BioMed Central Ltd. 2012. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.