High diversity of root associated fungi in both alpine and arctic Dryas octopetala

BMC Plant Biology

Research Article

Anne Brysting¹  Jozsef Geml²  Karl Inne Ugland³  Marit Frederikke Markussen Bjorbækmo⁴  Tor Carlsen⁴  Trude Vrålstad⁴  Trond Schumacher⁴  Håvard Kauserud⁴  Klaus Høiland⁴ 

[1] Centre for Ecological and Evolutionary Synthesis (CEES), Department of Biology, University of Oslo, P.O. Box 1066, N-0316, Blindern, Oslo, Norway;Kits van Waveren Foundation, Nationaal Herbarium Nederland, Universiteit Leiden, P.O. Box 9514, 2300, Leiden, RA, Nederland;Marine Biology, Department of Biology, University of Oslo, P.O. Box 1066, N-0316, Blindern, Oslo, Norway;Microbial Evolution Research Group (MERG), Department of Biology, University of Oslo, P.O. Box 1066, N-0316, Blindern, Oslo, Norway;

关键词: Root System;    Internal Transcribe Spacer;    Fungal Community;    Detrended Correspondence Analysis;    Fungal Diversity;   

DOI  :  10.1186/1471-2229-10-244

 received in 2010-07-07, accepted in 2010-11-11,  发布年份 2010

来源: Springer

【 摘 要 】

BackgroundDryas octopetala is a widespread dwarf shrub in alpine and arctic regions that forms ectomycorrhizal (ECM) symbiotic relationships with fungi. In this study we investigated the fungal communities associated with roots of D. octopetala in alpine sites in Norway and in the High Arctic on Svalbard, where we aimed to reveal whether the fungal diversity and species composition varied across the Alpine and Arctic regions. The internal transcribed spacer (ITS) region of nuclear ribosomal DNA was used to identify the fungal communities from bulk root samples obtained from 24 plants.ResultsA total of 137 operational taxonomic units (OTUs) were detected (using 97% similarity cut off during sequence clustering) and well-known ECM genera such as Cenococcum, Cortinarius, Hebeloma, Inocybe and Tomentella occurred frequently. There was no decrease in fungal diversity with increasing latitude. The overall spatial heterogeneity was high, but a weak geographical structuring of the composition of OTUs in the root systems was observed. Calculated species accumulation curves did not level off.ConclusionsThis study indicates that the diversity of fungi associated with D. octopetala does not decrease in high latitude arctic regions, which contrasts observations made in a wide spectrum of other organism groups. A high degree of patchiness was observed across root systems, but the fungal communities were nevertheless weakly spatially structured. Non-asymptotical species accumulation curves and the occurrence of a high number of singletons indicated that only a small fraction of the fungal diversity was detected.

【 授权许可】

Unknown   
© Bjorbækmo et al; licensee BioMed Central Ltd. 2010. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.