Comparative transcriptome analysis by RNAseq of necrotic enteritis Clostridium perfringens during in vivo colonization and in vitro conditions

BMC Microbiology

Research Article

Valeria R. Parreira¹  John F. Prescott¹  Kay Russell²  Spiridoula Athanasiadou² 

[1] Department of Pathobiology, University of Guelph, N1G 2W1, Guelph, ON, Canada;Disease Systems, Animal and Veterinary Sciences SRUC, Roslin Institute Building, EH25 9RG, Midlothian, Scotland;

关键词: Clostridium perfringens;    Transcriptome;    RNASeq;   

DOI  :  10.1186/s12866-016-0792-6

 received in 2016-05-01, accepted in 2016-07-28,  发布年份 2016

来源: Springer

【 摘 要 】

BackgroundNecrotic enteritis (NE) caused by netB-positive type A Clostridium perfringens is an important bacterial disease of poultry. Through its complex regulatory system, C. perfringens orchestrates the expression of a collection of toxins and extracellular enzymes that are crucial for the development of the disease; environmental conditions play an important role in their regulation. In this study, and for the first time, global transcriptomic analysis was performed on ligated intestinal loops in chickens colonized with a netB-positive C. perfringens strain, as well as the same strain propagated in vitro under various nutritional and environmental conditions.ResultsAnalysis of the respective pathogen transcriptomes revealed up to 673 genes that were significantly expressed in vivo. Gene expression profiles in vivo were most similar to those of C. perfringens grown in nutritionally-deprived conditions.ConclusionsTaken together, our results suggest a bacterial transcriptome responses to the early stages of adaptation, and colonization of, the chicken intestine. Our work also reveals how netB-positive C. perfringens reacts to different environmental conditions including those in the chicken intestine.

【 授权许可】

CC BY   
© The Author(s). 2016