A matched cross-sectional study of the association between circulating tissue factor activity, immune activation and advanced liver fibrosis in hepatitis C infection

BMC Infectious Diseases

Research Article

Weihua Gao¹  Kirsten E Brady²  Jill Plants²  Alan L Landay²  Stacey Kincaid³  Mieoak Bahk³  Gregory D Huhn⁴  Aimee C Hodowanec⁴  Rebecca D Lee⁵  Nigel Mackman⁵ 

[1] Center for Clinical and Translational Sciences, University of Illinois at Chicago, Chicago, IL, USA;Rush University Medical Center, Department of Immunology and Microbiology, Chicago, IL, USA;Ruth M. Rothstein CORE Center, 2020 W. Harrison St, 60612, Chicago, IL, USA;Ruth M. Rothstein CORE Center, 2020 W. Harrison St, 60612, Chicago, IL, USA;Rush University Medical Center, Section of Infectious Diseases, Chicago, IL, USA;University of North Carolina at Chapel Hill, Division of Hematology and Oncology, Chapel Hill, NC, USA;

关键词: Viral hepatitis;    Hepatitis C;    HIV;    Tissue factor;    Fibrosis;    Cirrhosis;    Immune activation;    Coagulation;   

DOI  :  10.1186/s12879-015-0920-1

 received in 2014-05-02, accepted in 2015-03-31,  发布年份 2015

来源: Springer

【 摘 要 】

BackgroundTissue factor (TF) is a protein that mediates the initiation of the coagulation cascade. TF expression is increased in patients with poorly-controlled HIV, and may be associated with increased immune activation that leads to cardiovascular morbidity. The role of TF in immune activation in liver disease in hepatitis C virus (HCV)-monoinfection and HIV/HCV-coinfection has not been explored.MethodsFifty-nine patients were stratified: A) HIV-monoinfection (N = 15), B) HCV-monoinfection with chronic hepatitis C (CHC) (N = 15), C) HIV/HCV-coinfection with CHC (N = 14), and D) HIV/HCV-seropositive with cleared-HCV (N = 15). All HIV+ patients had undetectable HIV viremia. Whole blood was collected for CD4/CD8 immune activation markers by flow cytometry and plasma was assayed for microparticle TF (MPTF) activity. Subjects underwent transient elastography (TE) to stage liver fibrosis. Undetectable versus detectable MPTF was compared across strata using Fisher's Exact test.ResultsMPTF activity was more frequently detected among patients with HCV-monoinfection (40%), compared to HIV-monoinfection and HIV/HCV-seropositive with cleared HCV (7%) and HIV/HCV-coinfection with CHC (14%) (p = 0.02). Mean TE-derived liver stiffness score in kPa was higher in patients with detectable MPTF (12.4 ± 8.5) than those with undetectable MPTF (6.4 ± 3.0) (p = 0.01). Mean CD4 + HLADR+ and CD4 + CD38-HLADR+ expression were higher in those with detectable MPTF (44 ± 9.8% and 38 ± 8.7%, respectively) than those with undetectable MPTF (36 ± 11% and 31 ± 10.4% respectively) (p = 0.05 and 0.04 respectively).ConclusionsHCV-monoinfection and HIV/HCV-coinfection with CHC were associated with MPTF activity. MPTF activity is also associated with advanced liver fibrosis and with CD4 + HLADR+ immune activation.

【 授权许可】

CC BY   
© Hodowanec et al.; licensee Biomed Central. 2015