Cardioprotection provided by Echinatin against ischemia/reperfusion in isolated rat hearts

BMC Cardiovascular Disorders

Research Article

Chao-liang Liu¹  Yan Zhang²  Meng Chen³  Hai-Li Jiang⁴  Xing-han Tian⁵  Ji-chun Han⁶  Ju Liu⁶ 

[1] Cardiovascular Department of Affiliated Hospital of JiNing Medical University, Jining, China;Department of Internal medicine, Qihe people’s hospital, Dezhou, China;Department of Internal medicine, Yantai Yuhuangding Hospital of Laishan branch, Yantai, China;Department of Oncology, Shuguang Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai, China;Intensive Care Unit, Yantai Yuhuangding Hospital of Laishan branch, Yantai, China;Medical Research Center, Shandong Provincial Qianfoshan Hospital, Shandong University, Jinan, China;

关键词: Echinatin;    Cardioprotection;    Ischemia/reperfusion;    Anti-inflammatory;    Antioxidant;   

DOI  :  10.1186/s12872-016-0294-3

 received in 2015-10-29, accepted in 2016-05-21,  发布年份 2016

来源: Springer

【 摘 要 】

BackgroundThis study evaluated the protective effect of Echinatin against myocardial ischemia/reperfusion (I/R) injury in rats.MethodsThe effect of Echinatin on cardiac function in rats subjected to I/R was demonstrated through improved Langendorff retrograde perfusion technology. Adult Sprague–Dawley rats were randomly divided into five groups, and myocardial infarct size was macroscopically estimated through 2,3,5-triphenyltetrazolium chloride staining. The coronary effluent was analyzed for the release of lactate dehydrogenase (LDH) and creatine kinase (CK) to assess the degree of cardiac injury. The concentrations of malondialdehyde (MDA), interleukin-6 (IL-6), and tumor necrosis factor-α (TNF-α) were determined along with superoxide dismutase (SOD) activity using ELISA. Finally, cardiomyocyte apoptosis analysis was conducted with POD, an in situ cell death detection kit.ResultsEchinatin (0.5 and 2.5 μg/mL) pretreatment enhanced the maximum up/down rate of the left ventricular pressure (±dp/dtmax), improved the heart rate, increased the left ventricular developed pressure (LVDP), enhanced the coronary flow, and reduced the CK and LDH levels in the coronary flow of the treated group compared with the I/R group. Echinatin limited the contents of CK and LDH, improved the LVDP, reduced the contents of MDA, IL-6, and TNF-α, and increased the SOD activity. The infarct size and cell apoptosis in the hearts of the rats in the Echinatin-treated group were smaller and lower, respectively, than those in the hearts of the rats in the I/R control group.ConclusionEchinatin exerts a protective effect against I/R-induced myocardial injury on hearts. This effect may be attributed to the antioxidant and anti-inflammatory activities of this compound.

【 授权许可】

CC BY   
© The Author(s). 2016