Kinotypes: stable species- and individual-specific profiles of cellular kinase activity

BMC Genomics

Research Article

Brett Trost¹  Anthony Kusalik¹  Jason Kindrachuk²  Erin Scruten³  Scott Napper⁴  Philip Griebel⁵ 

[1] Department of Computer Science, University of Saskatchewan, Saskatoon, Canada;Emerging Viral Pathogens Section, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Frederick, USA;Vaccine and Infectious Disease Organization, University of Saskatchewan, Saskatoon, Canada;Vaccine and Infectious Disease Organization, University of Saskatchewan, Saskatoon, Canada;Department of Biochemistry, University of Saskatchewan, Saskatoon, Canada;Vaccine and Infectious Disease Organization, University of Saskatchewan, Saskatoon, Canada;School of Public Health, University of Saskatchewan, Saskatoon, Canada;

关键词: Random Tree;    Peptide Array;    Variable Peptide;    Foreground Intensity;    Porcine Sample;   

DOI  :  10.1186/1471-2164-14-854

 received in 2013-08-28, accepted in 2013-11-29,  发布年份 2013

来源: Springer

【 摘 要 】

BackgroundRecently, questions have been raised regarding the ability of animal models to recapitulate human disease at the molecular level. It has also been demonstrated that cellular kinases, individually or as a collective unit (the kinome), play critical roles in regulating complex biology. Despite the intimate relationship between kinases and health, little is known about the variability, consistency and stability of kinome profiles across species and individuals.ResultsAs a preliminary investigation of the existence of species- and individual-specific kinotypes (kinome signatures), peptide arrays were employed for the analysis of peripheral blood mononuclear cells collected weekly from human and porcine subjects (n = 6) over a one month period. The data revealed strong evidence for species-specific signalling profiles. Both humans and pigs also exhibited evidence for individual-specific kinome profiles that were independent of natural changes in blood cell populations.ConclusionsSpecies-specific kinotypes could have applications in disease research by facilitating the selection of appropriate animal models or by revealing a baseline kinomic signature to which treatment-induced profiles could be compared. Similarly, individual-specific kinotypes could have implications in personalized medicine, where the identification of molecular patterns or signatures within the kinome may depend on both the levels of kinome diversity and temporal stability across individuals.

【 授权许可】

Unknown   
© Trost et al.; licensee BioMed Central Ltd. 2013. This article is published under license to BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.