Expression of human Piwi-likegenes is associated with prognosis for soft tissue sarcoma patients

BMC Cancer

Research Article

Thomas Greither¹  Peter Würl²  Franziska Koser³  Matthias Kappler³  Matthias Bache⁴  Sven Wach⁵  Helge Taubert⁶  Christine Lautenschläger⁷  Hans-Jürgen Holzhausen⁸  Steffen Göbel⁹ 

[1] Center for Reproductive Medicine and Andrology, Martin-Luther-University Halle-Wittenberg, Halle, Germany;Department of General and Visceral Surgery, Diakoniekrankenhaus Halle, Halle, Germany;Department of Oral and Maxillofacial Plastic Surgery, Martin-Luther-University Halle-Wittenberg, Halle, Germany;Department of Radiotherapy, Martin-Luther-University Halle-Wittenberg, Halle, Germany;Div. Molecular Urology, FAU Erlangen-Nürnberg, University Clinic of Urology, Erlangen, Germany;Nikolaus-Fiebiger-Center for Molecular Medicine, FAU Erlangen-Nürnberg, Erlangen, Germany;Div. Molecular Urology, FAU Erlangen-Nürnberg, University Clinic of Urology, Erlangen, Germany;Nikolaus-Fiebiger-Center for Molecular Medicine, FAU Erlangen-Nürnberg, Erlangen, Germany;Klinik für Urologie und NFZ, FAU Erlangen, Glückstr. 6, D-91054, Erlangen, Germany;Institute of Medical Biometry and Informatics, Martin-Luther-University Halle-Wittenberg, Halle, Germany;Institute of Pathology, Martin-Luther-University Halle-Wittenberg, Halle, Germany;Institute of Transfusion Medicine, Martin-Luther-University Halle-Wittenberg, Halle, Germany;

关键词: mRNA Expression Level;    Soft Tissue Sarcoma;    Hypoxanthine Phosphoribosyl Transferase;    Cervical Squamous Cell Carcinoma;    PIWI Protein;   

DOI  :  10.1186/1471-2407-12-272

 received in 2011-11-22, accepted in 2012-05-15,  发布年份 2012

来源: Springer

【 摘 要 】

BackgroundArgonaute genes are essential for RNA interference, stem cell maintenance and differentiation. The Piwi-like genes, a subclass of the Argonaute genes, are expressed mainly in the germline. These genes may be re-expressed in tumors, and expression of the Piwi-like genes is associated with prognosis in several types of tumors.MethodsWe measured the expression of Piwi-like mRNAs (Piwi-like 2–4) in 125 soft tissue sarcoma (STS) samples by qPCRs. Statistical tests were applied to study the correlation of expression levels with tumor-specific survival for STS patients.ResultsIn multivariate Cox’s regression analyses, we showed that low Piwi-like 2 and Piwi-like 4 mRNA expression were significantly associated with a worse prognosis (RR = 1.87; p = 0.032 and RR = 1.82; p = 0.039). Low expression of both genes was associated with a 2.58-fold increased risk of tumor-related death (p = 0.01). Piwi-like 4 and combined Piwi-like 2 and 4 mRNA levels correlated significantly with prognosis (RR = 3.53; p = 0.002 and RR = 5.23; p = 0.004) only for female but not for male patients. However, combined low Piwi-like 2 and 3 transcript levels were associated with worse survival (RR = 5.90; p = 0.02) for male patients.ConclusionsIn this study, we identified a significant association between the expression of Piwi-like 2 and 4 mRNAs and the tumor-specific survival of soft tissue sarcoma patients. Furthermore, a connection between sex and the impact of Piwi-like mRNA expressions on STS patients’ prognosis was shown for the first time.

【 授权许可】

Unknown   
© Greither et al.; licensee BioMed Central Ltd. 2012. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.