An experimental study on cervix cancer with combination of HSV-TK/GCV suicide gene therapy system and 60Co radiotherapy

BMC Cancer

Research Article

Daozhen Chen¹  Qiusha Tang² 

[1] Clinical Laboratory, Wuxi Hospital for Matemaland Child Health Care Affiliated Nanjing Medical University, 214002, Wuxi, Jiangsu, China;School of Clinical Medical Science, Southeast University, 210009, Nanjing, China;

关键词: Radiotherapy Group;    Suicide Gene;    Combine Treatment Group;    Absorption Dose;    Suicide Gene Therapy;   

DOI  :  10.1186/1471-2407-10-609

 received in 2010-07-01, accepted in 2010-11-06,  发布年份 2010

来源: Springer

【 摘 要 】

BackgroundTo evaluate the killing effect of HSV-TK/GCV suicide gene therapy system combined with 60Co radiotherapy on human cervical cancer Hela cell line in vitro and in vivo, and to explore the radiosensitization by HSV-TK/GCV system.MethodsHSV-TK/GCV suicide gene therapy system and 60Co radiotherapy were used separately or in combination on human cervical cancer Hela cell line in vitro and in vivo to compare their effects. Colony formation test and the rate of radiosensitization effect (E/O) were employed to observed the radiosensitization by HSV-TK/GCV system.ResultsHSV-TK/GCV suicide gene therapy system had strong therapeutic effects on Hela cells in vitro and in vivo (the inhibition rates were 45.8% and 39.5%, respectively), moreover, the combined administration of gene therapy and radiotherapy had stronger therapeutic effects in vitro and in vivo (the inhibition rate was 87.5% in vitro, and the inhibition rate was 87.9% in vivo) (P < 0.01). The inhibition rate by radiotherapy alone was 42.4% in vitroand 35.8% in vivo. The sensitivity of combined therapy to radiotherapy increased more than that of therapy alone, the ability of colony formation decreased (P < 0.01). The rate of radiosensitivity effect (E/O) was 3.17(> 1.4), indicating HSV-TK/GCV system could exert a sensitizing effect on 60Co radiotherapy of the transplanted human cervical cancer cell in nude mice.ConclusionHSV-TK/GCV system had radiosensitization. Gene therapy combined with radiotherapy may be a good supplementary method for cervix cancer synthetic treatment.

【 授权许可】

CC BY   
© Chen and Tang; licensee BioMed Central Ltd. 2010