A candidate gene association study on muscat flavor in grapevine (Vitis viniferaL.)

BMC Plant Biology

Research Article

Jean-Michel Boursiquot¹  Patrice This¹  Loïc Le Cunff²  Maria S Grando³  Francesco Emanuelli³  Laura Costantini³  Juri Battilana³ 

[1] INRA UMR 1097 Diversité et Adaptation des Plantes Cultivées, 34060, 2 place Viala, Montpellier, France;INRA UMR 1097 Diversité et Adaptation des Plantes Cultivées, 34060, 2 place Viala, Montpellier, France;UMT GenoVigne®, IFV-INRA-Montpellier Supagro, 34060, 2 place Viala, Montpellier, France;Research and Innovation Center, Fondazione Edmund Mach-Istituto Agrario di San Michele all'Adige, San Michele all'Adige, 38010, Via E. Mach 1, (TN), Italy;

关键词: Linkage Disequilibrium;    Table Grape;    Amino Acid Exchange;    Neutral Variety;    Reconstructed Haplotype;   

DOI  :  10.1186/1471-2229-10-241

 received in 2010-06-22, accepted in 2010-11-09,  发布年份 2010

来源: Springer

【 摘 要 】

BackgroundThe sweet, floral flavor typical of Muscat varieties (Muscats), due to high levels of monoterpenoids (geraniol, linalool and nerol), is highly distinct and has been greatly appreciated both in table grapes and in wine since ancient times. Muscat flavor determination in grape (Vitis vinifera L.) has up to now been studied by evaluating monoterpenoid levels through QTL analysis. These studies have revealed co-localization of 1-deoxy-D-xylulose 5-phosphate synthase (VvDXS) with the major QTL positioned on chromosome 5.ResultsWe resequenced VvDXS in an ad hoc association population of 148 grape varieties, which included muscat-flavored, aromatic and neutral accessions as well as muscat-like aromatic mutants and non-aromatic offsprings of Muscats. Gene nucleotide diversity and intragenic linkage disequilibrium (LD) were evaluated. Structured association analysis revealed three SNPs in moderate LD to be significantly associated with muscat-flavored varieties. We identified a putative causal SNP responsible for a predicted non-neutral substitution and we discuss its possible implications for flavor metabolism. Network analysis revealed a major star-shaped cluster of reconstructed haplotypes unique to muscat-flavored varieties. Moreover, muscat-like aromatic mutants displayed unique non-synonymous mutations near the mutated site of Muscat genotypes.ConclusionsThis study is a crucial step forward in understanding the genetic regulation of muscat flavor in grapevine and it also sheds light on the domestication history of Muscats. VvDXS appears to be a possible human-selected locus in grapevine domestication and post-domestication. The putative causal SNP identified in Muscat varieties as well as the unique mutations identifying the muscat-like aromatic mutants under study may be immediately applied in marker-assisted breeding programs aimed at enhancing fragrance and aroma complexity respectively in table grape and wine cultivars.

【 授权许可】

CC BY   
© Emanuelli et al; licensee BioMed Central Ltd. 2010