Thermal manipulation of the chicken embryo triggers differential gene expression in response to a later heat challenge

BMC Genomics

Research Article

Jérôme Lecardonnel¹  Marco Moroldo¹  Patrice Martin²  Shlomo Yahav³  Sandrine Lagarrigue⁴  Vincent Coustham⁵  Cécile Berri⁵  Sandrine Mignon-Grasteau⁵  Aurélien Brionne⁵  Marie Leduc⁵  Sabine Crochet⁵  Christelle Hennequet-Antier⁵  Sonia Métayer-Coustard⁵  Thomas Loyau⁵  Mélanie Sannier⁵  Anne Collin⁵  Sophie Tesseraud⁵  Michel Jacques Duclos⁵ 

[1] CRB GADIE, INRA, Domaine de Vilvert, 78350, Jouy-en-Josas, France;GABI, INRA, Plateforme de Microgénomique Iso Cell Express (ICE), 78350, Jouy-en-Josas, France;Institute of Animal Science, The Volcani Center, P.O. Box 6, 50250, Bet Dagan, Israel;PEGASE, INRA, Agrocampus Rennes, 35590, Saint-Gilles, France;URA, INRA, 37380, Nouzilly, France;

关键词: Brain Derive Neurotrophic Factor;    COQ6;    Differentially Express;    Ingenuity Pathway Analysis;    Differentially Express Gene;   

DOI  :  10.1186/s12864-016-2661-y

 received in 2016-02-05, accepted in 2016-04-25,  发布年份 2016

来源: Springer

【 摘 要 】

BackgroundMeat type chickens have limited capacities to cope with high environmental temperatures, this sometimes leading to mortality on farms and subsequent economic losses. A strategy to alleviate this problem is to enhance adaptive capacities to face heat exposure using thermal manipulation (TM) during embryogenesis. This strategy was shown to improve thermotolerance during their life span. The aim of this study was to determine the effects of TM (39.5 °C, 12 h/24 vs 37.8 °C from d7 to d16 of embryogenesis) and of a subsequent heat challenge (32 °C for 5 h) applied on d34 on gene expression in the Pectoralis major muscle (PM). A chicken gene expression microarray (8 × 60 K) was used to compare muscle gene expression profiles of Control (C characterized by relatively high body temperatures, Tb) and TM chickens (characterized by a relatively low Tb) reared at 21 °C and at 32 °C (CHC and TMHC, respectively) in a dye-swap design with four comparisons and 8 broilers per treatment. Real-time quantitative PCR (RT-qPCR) was subsequently performed to validate differential expression in each comparison. Gene ontology, clustering and network building strategies were then used to identify pathways affected by TM and heat challenge.ResultsAmong the genes differentially expressed (DE) in the PM (1.5 % of total probes), 28 were found to be differentially expressed between C and TM, 128 between CHC and C, and 759 between TMHC and TM. No DE gene was found between TMHC and CHC broilers. The majority of DE genes analyzed by RT-qPCR were validated. In the TM/C comparison, DE genes were involved in energy metabolism and mitochondrial function, cell proliferation, vascularization and muscle growth; when comparing heat-exposed chickens to their own controls, TM broilers developed more specific pathways than C, especially involving genes related to metabolism, stress response, vascularization, anti-apoptotic and epigenetic processes.ConclusionsThis study improved the understanding of the long-term effects of TM on PM muscle. TM broilers displaying low Tb may have lower metabolic intensity in the muscle, resulting in decreased metabolic heat production, whereas modifications in vascularization may enhance heat loss. These specific changes could in part explain the better adaptation of TM broilers to heat.

【 授权许可】

CC BY   
© Loyau et al. 2016