Protective activity of Panduratin A against Thioacetamide-induced oxidative damage: demonstration with in vitro experiments using WRL-68 liver cell line

BMC Complementary and Alternative Medicine

Research Article

Mehmet Bilgen¹  Ahmed S AlRashdi²  Pouya Hassandarvish²  Mahmood A Abdulla²  Suzy M Salama² 

[1] Biophysics Department, Faculty of Medicine, Erciyes University, 38039, Kayseri, Turkey;Department of Molecular Medicine and Biomedical Science, Faculty of Medicine, University of Malaya, 50603, Kuala Lumpur, Malaysia;

关键词: Cytotoxicity;    Hepatocyte;    Hepatoprotection;    Boesenbergia rotunda;    Panduratin A;    WRL-68 liver cell line;    Oxidative stress;   

DOI  :  10.1186/1472-6882-13-279

 received in 2013-06-22, accepted in 2013-10-17,  发布年份 2013

来源: Springer

【 摘 要 】

BackgroundChalcone Panduratin A (PA) has been known for its antioxidant property, but its merits against oxidative damage in liver cells has yet to be investigated. Hence, the paper aimed at accomplishing this task with normal embryonic cell line WRL-68.MethodsPA was isolated from Boesenbergia rotunda rhizomes and its 2,2-diphenyl-1-picrylhydrazyl (DPPH) scavenging and ferric reducing power (FRAP) activities were measured in comparison with that of the standard reference drug Silymarin (SI). Oxidative damage was induced by treating the cells with 0.04 g/ml of toxic thioacetamide for 60 minutes followed by treatment with 1, 10 and 100 μg/ml concentrations of either PA or SI. The severities of oxidative stress in the control and experimental groups of cells were measured by Malondialdehyde (MDA) levels, superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPx) activities.ResultsPA exhibited an acceptable DPPH scavenging and FRAP activities close to that of Silymarin. Treating the injured cells with PA significantly reduced the MDA level and increased the cell viability, comparable to SI. The activities of SOD, CAT and GPx were significantly elevated in the PA-treated cells in a dose dependent manner and again similar to SI.ConclusionCollectively, data suggested that PA has capacity to protect normal liver cells from oxidative damage, most likely via its antioxidant scavenging ability.

【 授权许可】

Unknown   
© Salama et al.; licensee BioMed Central Ltd. 2013. This article is published under license to BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.