Centriole movements in mammalian epithelial cells during cytokinesis

BMC Cell Biology

Research Article

Helga M Ögmundsdottir¹  Jorunn E Eyfjörd¹  Asta Björk Jonsdottir²  Johannes Vrolijk³  Karoly Szuhai³  Roeland W Dirks³  Hans J Tanke³ 

[1] Cancer Research Laboratory, Faculty of Medicine, University of Iceland, Vatnsmyrarvegi 16, 101, Reykjavik, Iceland;Cancer Research Laboratory, Faculty of Medicine, University of Iceland, Vatnsmyrarvegi 16, 101, Reykjavik, Iceland;Department of Molecular Cell Biology, Leiden University Medical Center, Einthovenweg 20, 2300, Leiden, RC, the Netherlands;Department of Molecular Cell Biology, Leiden University Medical Center, Einthovenweg 20, 2300, Leiden, RC, the Netherlands;

关键词: HeLa Cell;    Nuclear Envelope;    Daughter Cell;    Emerin;    Spindle Pole Body;   

DOI  :  10.1186/1471-2121-11-34

 received in 2009-12-22, accepted in 2010-05-21,  发布年份 2010

来源: Springer

【 摘 要 】

BackgroundIn cytokinesis, when the cleavage furrow has been formed, the two centrioles in each daughter cell separate. It has been suggested that the centrioles facilitate and regulate cytokinesis to some extent. It has been postulated that termination of cytokinesis (abscission) depends on the migration of a centriole to the intercellular bridge and then back to the cell center. To investigate the involvement of centrioles in cytokinesis, we monitored the movements of centrioles in three mammalian epithelial cell lines, HeLa, MCF 10A, and the p53-deficient mouse mammary tumor cell line KP-7.7, by time-lapse imaging. Centrin1-EGFP and α-Tubulin-mCherry were co-expressed in the cells to visualize respectively the centrioles and microtubules.ResultsHere we report that separated centrioles that migrate from the cell pole are very mobile during cytokinesis and their movements can be characterized as 1) along the nuclear envelope, 2) irregular, and 3) along microtubules forming the spindle axis. Centriole movement towards the intercellular bridge was only seen occasionally and was highly cell-line dependent.ConclusionsThese findings show that centrioles are highly mobile during cytokinesis and suggest that the repositioning of a centriole to the intercellular bridge is not essential for controlling abscission. We suggest that centriole movements are microtubule dependent and that abscission is more dependent on other mechanisms than positioning of centrioles.

【 授权许可】

CC BY   
© Jonsdottir et al; licensee BioMed Central Ltd. 2010