Transcriptome based identification of mouse cumulus cell markers that predict the developmental competence of their enclosed antral oocytes

BMC Genomics

Research Article

Francesca Mulas¹  Riccardo Bellazzi²  Alessandro Prigione³  James Adjaye⁴  Matteo Gabetta⁵  Lucia Sacchi⁵  Maurizio Zuccotti⁶  Giuliano Mazzini⁷  Giulia Vigone⁸  Valeria Merico⁸  Carlo Alberto Redi⁸  Silvia Garagna⁹ 

[1] Centro di Ingegneria Tissutale, Universita’ degli Studi di Pavia, Pavia, Italy;Centro di Ingegneria Tissutale, Universita’ degli Studi di Pavia, Pavia, Italy;Dipartimento di Ingegneria Industriale e dell’Informazione, Universita’ degli Studi di Pavia, Pavia, Italy;Department of Vertebrate Genomics. Max-Planck Institute for Molecular Genetics, Molecular Embryology and Aging Group, Berlin, Germany;Department of Vertebrate Genomics. Max-Planck Institute for Molecular Genetics, Molecular Embryology and Aging Group, Berlin, Germany;Institute for Stem Cell Research and Regenerative Medicine, Medical Faculty, Heinrich Heine University, Düsseldorf, Germany;Dipartimento di Ingegneria Industriale e dell’Informazione, Universita’ degli Studi di Pavia, Pavia, Italy;Dipartimento di Scienze Biomediche, Biotecnologiche e Traslazionali (S.Bi.Bi.T.), Sezione di Anatomia, Istologia ed Embriologia, Universita’ degli Studi di Parma, Via Volturno, 39-43125, Parma, Italy;Istituto di Genetica Molecolare – Consiglio Nazionale delle Ricerche, Via Ferrata 9, Pavia, Italy;Laboratorio di Biologia dello Sviluppo, Dipartimento di Biologia e Biotecnologie ‘Lazzaro Spallanzani’, Universita’ degli Studi di Pavia, Pavia, Italy;Laboratorio di Biologia dello Sviluppo, Dipartimento di Biologia e Biotecnologie ‘Lazzaro Spallanzani’, Universita’ degli Studi di Pavia, Pavia, Italy;Centro di Ingegneria Tissutale, Universita’ degli Studi di Pavia, Pavia, Italy;Centro di Eccellenza in Biologia Applicata, Universita’ degli Studi di Pavia, Pavia, Italy;

关键词: Oocyte;    Developmental competence;    Cumulus cells;    Transcriptome;   

DOI  :  10.1186/1471-2164-14-380

 received in 2013-02-20, accepted in 2013-05-30,  发布年份 2013

来源: Springer

【 摘 要 】

BackgroundThe cumulus cells (CCs) enveloping antral and ovulated oocytes have been regarded as putative source of non-invasive markers of the oocyte developmental competence. A number of studies have indeed observed a correlation between CCs gene expression, embryo quality, and final pregnancy outcome. Here, we isolated CCs from antral mouse oocytes of known developmental incompetence (NSN-CCs) or competence (SN-CCs) and compared their transcriptomes with the aim of identifying distinct marker transcripts.ResultsGlobal gene expression analysis highlighted that both types of CCs share similar transcriptomes, with the exception of 422 genes, 97.6% of which were down-regulated in NSN-CCs vs. SN-CCs. This transcriptional down-regulation in NSN-CCs was confirmed by qRT-PCR analysis of CC-related genes (Has2, Ptx3, Tnfaip6 and Ptgs2). Only ten of the 422 genes were up-regulated with Amh being the most up-regulated in NSN-CCs, with an average 4-fold higher expression when analysed by qRT-PCR.ConclusionsThe developmental incompetence (NSN) or competence (SN) of antral oocytes can be predicted using transcript markers expressed by their surrounding CCs (i.e., Has2, Ptx3, Tnfaip6, Ptgs2 and Amh). Overall, the regulated nature of the group of genes brought out by whole transcriptome analysis constitutes the molecular signature of CCs associated either with developmentally incompetent or competent oocytes and may represent a valuable resource for developing new molecular tools for the assessment of oocyte quality and to further investigate the complex bi-directional interaction occurring between CCs and oocyte.

【 授权许可】

Unknown   
© Vigone et al.; licensee BioMed Central Ltd. 2013. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.