Metabolomic investigation of regional brain tissue dysfunctions induced by global cerebral ischemia

BMC Neuroscience

Research Article

Wei Wang¹  Haiyan Zhang¹  Naixia Zhang²  Xia Liu²  Tianshu Zhang³  Jin Huang³ 

[1] CAS Key Laboratory of Receptor Research, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, 201203, Shanghai, China;Department of Analytical Chemistry, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, 201203, Shanghai, China;Shanghai Key Laboratory of New Drug Design, School of Pharmacy, East China University of Science and Technology, 200237, Shanghai, China;

关键词: Bilateral common carotid arteries occlusion;    Metabolomics;    NMR;    Brain tissue dysfunctions;    Amino acid metabolism;   

DOI  :  10.1186/s12868-016-0256-9

 received in 2015-08-20, accepted in 2016-05-11,  发布年份 2016

来源: Springer

【 摘 要 】

BackgroundTo get a broader view of global ischemia-induced cerebral disorders at the metabolic level, a nuclear magnetic resonance-based metabolomic study was performed to evaluate the metabolic profile changes on regional brain tissues of female and male mice upon bilateral common carotid arteries occlusion (BCCAO) operation.ResultsSignificant metabolic disorders were observed in both cerebral cortex and hippocampus tissues of the experimental mice upon global cerebral ischemic attack. Multiple amino acids were identified as the dominantly perturbed metabolites. It was also shown that although the metabolic profile change patterns in the brain tissues were quite similar in male and female BCCAO mice, metabolic disorders in the cortex tissues were more severe in the female mice than in the male mice.ConclusionsIn the present study, significant changes in amino acid metabolic pathways were confirmed in the early stage of global ischemia. Meanwhile, cerebral metabolic dysfunctions were more severe in the female BCCAO mice than in the male mice, suggesting that gender may play a role in different metabolic responses to the ischemic attack, which may provide an important hypothesis for a better understanding of the clinically observed gender-dependent pathological outcome of cerebral ischemia.

【 授权许可】

CC BY   
© The Author(s). 2016