High frequency of pre-existing neutralizing antibody responses in patients with dengue during an outbreak in Central Brazil

BMC Infectious Diseases

Research Article

Wayner Vieira de Souza¹  Marli Tenório Cordeiro¹  Ernesto Torres de Azevedo Marques²  Lucimeire Antonelli da Silveira³  Adriana Oliveira Guilarde³  Celina Maria Turchi Martelli⁴  Angela Ferreira Lopes de Teive e Argolo⁵  Valéria Christina de Rezende Féres⁶ 

[1] Centro de Pesquisas Aggeu Magalhães da Fundação Oswaldo, Av. Prof. Moraes Rego, 1235, Cidade Universitária, 50.670-901, Recife, PE, Brazil;Centro de Pesquisas Aggeu Magalhães da Fundação Oswaldo, Av. Prof. Moraes Rego, 1235, Cidade Universitária, 50.670-901, Recife, PE, Brazil;Center for Vaccine Research, Department of Infectious Diseases and Microbiology, University of Pittsburgh, Pittsburgh, PA, USA;Institute of Tropical Pathology and Public Health of Federal University of Goiás, Rua 235, St Universitário, 74.605-050, Goiânia, GO, Brazil;Institute of Tropical Pathology and Public Health of Federal University of Goiás, Rua 235, St Universitário, 74.605-050, Goiânia, GO, Brazil;Centro de Pesquisas Aggeu Magalhães da Fundação Oswaldo, Av. Prof. Moraes Rego, 1235, Cidade Universitária, 50.670-901, Recife, PE, Brazil;Institute of Tropical Pathology and Public Health of Federal University of Goiás, Rua 235, St Universitário, 74.605-050, Goiânia, GO, Brazil;Public Health Laboratory of Secretaria Estadual de Saúde de Goiás (LACEN-GO), Av. Contorno, 3.556, Jd. Bela Vista, 74.853-120, Goiânia, GO, Brazil;Pharmacy College of Federal University of Goiás, Av. Universitaria, St. Universitario, 74.605-220, Goiânia, GO, Brazil;

关键词: Dengue virus;    Neutralizing antibody;    Dengue fever;    Brazil;   

DOI  :  10.1186/s12879-016-1867-6

 received in 2015-10-31, accepted in 2016-09-24,  发布年份 2016

来源: Springer

【 摘 要 】

BackgroundThis study aims to identify dengue neutralizing antibody response in patients with dengue from a well-characterized cohort during an outbreak in central Brazil, 2012–2013.MethodsWe analyzed paired samples from 40 patients with severe dengue and 20 patients with dengue. Eligibility criteria were: IgM, NS1Ag and/or RT-PCR positivity and positive IgG result. Plaque reduction neutralization test (PRNT50) from DENV-1 to DENV-4 was performed to identify serotype-specific NAbs response. An infecting serotype was defined as ≥4-fold increase in DENV NAbs in paired samples. Monotypic response was classified as PRNT50 ≥ 1/20 to only one DENV serotype, and multitypic response was considered to be PRNT50 ≥ 1/20 to two or more serotypes simultaneously.ResultsPatients were mainly adults. Virological dengue infection was confirmed by RT-PCR: DENV-4(n = 14) and DENV-1(n = 10). Forty-four out of 60(73.3 %) patients had NAbs to DENV-4, DENV-1(68.3 %), DENV-2(68.3 %) and DENV-3(61.6 %) respectively. Fifteen percent of the cases presented monotypic response, whereas 85 % had multitypic response. DENV-4 infected-patients presented the greatest difference in PRNT50 titers compared with other serotypes. Pre-existing DENV NAbs was not correlated with disease severity. This was the first time that DENV-4 was implicated in an epidemic in the region.ConclusionOur data indicates high exposure of multiple DENV serotypes in all age groups in the pre-dengue vaccine era and also previous to Zika virus introduction in Brazil.

【 授权许可】

CC BY   
© The Author(s). 2016