Escherichia coli infection induces distinct local and systemic transcriptome responses in the mammary gland

BMC Genomics

Research Article

Holm Zerbe¹  Wolfram Petzl¹  Daniel Mehne¹  Simone Mitterhuemer²  Andrea Klanner²  Stefan Krebs²  Helmut Blum²  Eckhard Wolf³ 

[1] Clinic for Ruminants, Center for Clinical Veterinary Medicine, LMU Munich, Sonnenstr 16, 85764, Oberschleißheim, Germany;Laboratory for Functional Genome Analysis (LAFUGA), Gene Center, LMU Munich, Feodor-Lynen-Str 25, 81377, Munich, Germany;Laboratory for Functional Genome Analysis (LAFUGA), Gene Center, LMU Munich, Feodor-Lynen-Str 25, 81377, Munich, Germany;Chair for Molecular Animal Breeding and Biotechnology, Gene Center, LMU Munich, Feodor-Lynen-Str 25, 81377, Munich, Germany;

关键词: Mammary Gland;    Mastitis;    Milk Yield;    Differentially Express Gene;    Somatic Cell Count;   

DOI  :  10.1186/1471-2164-11-138

 received in 2009-06-30, accepted in 2010-02-25,  发布年份 2010

来源: Springer

【 摘 要 】

BackgroundColiform bacteria are the most common etiologic agents in severe mastitis of cows. Escherichia coli infections are mostly restricted to a single udder quarter whereas neighboring quarters stay clinically inapparent, implicating the presence of a systemic defense reaction. To address its underlying mechanism, we performed a transcriptome study of mammary tissue from udder quarters inoculated with E. coli (6 h and 24 h post infection), from neighboring quarters of the same animals, and from untreated control animals.ResultsAfter 6 h 13 probe sets of differentially expressed genes (DEG) were detected in infected quarters versus control animals. Eighteen hours later 2154 and 476 DEG were found in infected and in neighboring quarters vs. control animals. Cluster analysis revealed DEG found only in infected quarters (local response) and DEG detected in both infected and neighboring quarters (systemic response). The first group includes genes mainly involved in immune response and inflammation, while the systemic reaction comprises antigen processing and presentation, cytokines, protein degradation and apoptosis. Enhanced expression of antimicrobial genes (S100A8, S100A9, S100A12, CXCL2, GNLY), acute phase genes (LBP, SAA3, CP, BF, C6, C4BPA, IF), and indicators of oxidative stress (GPX3, MT1A, MT2A, SOD2) point to an active defense reaction in infected and neighboring healthy quarters. Its early onset is indicated by increased transcription of NFIL3 at 6 h. NFIL3 is a predicted regulator of many genes of the systemic response at 24 h. The significance of our transcriptome study was evidenced by some recent findings with candidate gene based approaches.ConclusionsThe discovery and holistic analysis of an extensive systemic reaction in the mammary gland significantly expands the knowledge of host-pathogen interactions in mastitis which may be relevant for the development of novel therapies and for genetic selection towards mastitis resistance.

【 授权许可】

Unknown   
© Mitterhuemer et al; licensee BioMed Central Ltd. 2010. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.