Smoking decreases the level of circulating CD34+ progenitor cells in young healthy women - a pilot study

BMC Women's Health

Research Article

Christine Gericke¹  Verena Stangl²  Karl Stangl²  Claudia Kuhn²  Simone Mueller²  Gert Baumann²  Antje Ludwig²  Andras Kertesz²  Nicoline Jochmann² 

[1] Institut für Biometrie und Klinische Epidemiologie, Charité - Universitaetsmedizin Berlin, Campus Mitte, Berlin, Germany;Med. Klinik mit Schwerpunkt Kardiologie und Angiologie, Charité - Universitaetsmedizin Berlin, Campus Mitte, Berlin, Germany;

关键词: Progenitor Cell;    Menstrual Cycle;    Endothelial Function;    Menstrual Bleeding;    Menstrual Phase;   

DOI  :  10.1186/1472-6874-10-20

 received in 2009-10-13, accepted in 2010-05-30,  发布年份 2010

来源: Springer

【 摘 要 】

BackgroundDecreased levels of circulating bone marrow-derived progenitor cells have been associated with risk factors and cardiovascular diseases. Smoking is the most important modifiable risk factor for atherosclerosis in young women. The aim of this pilot study was to assess in healthy premenopausal women without other risk factors for cardiovascular disease the influence of nicotine abuse on the number of circulating progenitor cells in relation to endothelial function.MethodsThe number of endothelial progenitor cells, measured as colony-forming units in a cell-culture assay (EPC-CFU) and the number of circulating CD34 + and CD34 + /CD133 + cells, measured by flow cytometry, was estimated in 32 women at the menstrual phase of the menstrual cycle. In addition, flow-mediated dilation (FMD) was assessed as a marker for vascular function. In a subgroup of these women (n = 20), progenitor cells were also investigated at the mid-follicular and luteal phases of the menstrual cycle.ResultsCompared to non-smokers, the abundance of circulating CD34 + cells was significantly lower in smoking women in the menstrual, mid-luteal, and mid-follicular phases of the menstrual cycle. The number of CD34 + progenitor cells was revealed to have significant positive correlation with FMD in young healthy women, whereas CD34 + /CD133 + progenitor cells and EPC-CFU showed no significant correlation.ConclusionThe number of CD34 + progenitor cells positively correlates with FMD in young healthy women and is decreased by smoking.

【 授权许可】

Unknown   
© Ludwig et al; licensee BioMed Central Ltd. 2010. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.